WO2001092291A3 - Antibody and t-cell receptor libraries - Google Patents

Antibody and t-cell receptor libraries Download PDF

Info

Publication number
WO2001092291A3
WO2001092291A3 PCT/IL2001/000496 IL0100496W WO0192291A3 WO 2001092291 A3 WO2001092291 A3 WO 2001092291A3 IL 0100496 W IL0100496 W IL 0100496W WO 0192291 A3 WO0192291 A3 WO 0192291A3
Authority
WO
WIPO (PCT)
Prior art keywords
antibody
cell receptor
cell
genes
receptor libraries
Prior art date
Application number
PCT/IL2001/000496
Other languages
French (fr)
Other versions
WO2001092291A2 (en
Inventor
Gideon Gross
Yaaqov Schlesinger
Original Assignee
Galim Galil Immunology Ltd
Gideon Gross
Yaaqov Schlesinger
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Galim Galil Immunology Ltd, Gideon Gross, Yaaqov Schlesinger filed Critical Galim Galil Immunology Ltd
Priority to AU62636/01A priority Critical patent/AU6263601A/en
Publication of WO2001092291A2 publication Critical patent/WO2001092291A2/en
Publication of WO2001092291A3 publication Critical patent/WO2001092291A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid

Abstract

The invention relates to RNA trans-splicing-mediated covalent intracellular fusion of transcripts of two different genes A and B in a cell, particularly in an immune cell, in which said genes A and B are expressible, and to DNA constructs encoding RNAs useful in such processes. The genes A and B may encode the light and heavy chains of an antibody or the α and β chains of a T-cell receptor. This process leads to a unique and novel approach for creating immune antibody and T-cell receptor libraries.
PCT/IL2001/000496 2000-05-30 2001-05-30 Antibody and t-cell receptor libraries WO2001092291A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU62636/01A AU6263601A (en) 2000-05-30 2001-05-30 Antibody and t-cell receptor libraries

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL136459 2000-05-30
IL13645900A IL136459A0 (en) 2000-05-30 2000-05-30 Antibody library

Publications (2)

Publication Number Publication Date
WO2001092291A2 WO2001092291A2 (en) 2001-12-06
WO2001092291A3 true WO2001092291A3 (en) 2002-05-16

Family

ID=11074201

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2001/000496 WO2001092291A2 (en) 2000-05-30 2001-05-30 Antibody and t-cell receptor libraries

Country Status (3)

Country Link
AU (1) AU6263601A (en)
IL (1) IL136459A0 (en)
WO (1) WO2001092291A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9181527B2 (en) 2009-10-29 2015-11-10 The Trustees Of Dartmouth College T cell receptor-deficient T cell compositions

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003054021A2 (en) * 2001-12-20 2003-07-03 Agricultural Research Council Semi-synthetic antibody fragment combinatorial library based on avian immunoglobulin genes
ES2327229T3 (en) 2002-11-09 2009-10-27 Immunocore Ltd. PRESENTATION OF THE RECEIVER OF LYMPHOCYTES T.
TWI333977B (en) 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
GB0411771D0 (en) * 2004-05-26 2004-06-30 Avidex Ltd Nucleoproteins displaying native T cell receptor libraries
US7994298B2 (en) 2004-09-24 2011-08-09 Trustees Of Dartmouth College Chimeric NK receptor and methods for treating cancer
WO2006042232A2 (en) * 2004-10-08 2006-04-20 Intronn, Inc. Targeted trans-splicing of highly abundant transcripts for in vivo production of recombinant proteins
WO2006083331A2 (en) * 2004-10-08 2006-08-10 Intronn, Inc Use of rna trans-splicing for antibody gene transfer and antibody polypeptide production
ES2573027T3 (en) 2006-05-17 2016-06-03 F. Hoffmann-La Roche Ag Polypeptide producing cells
PT2121920E (en) 2007-03-01 2011-10-03 Symphogen As Method for cloning cognate antibodies
DK2435568T3 (en) 2009-05-29 2014-09-08 Morphosys Ag Collection of synthetic antibodies to treat disease
US9273283B2 (en) 2009-10-29 2016-03-01 The Trustees Of Dartmouth College Method of producing T cell receptor-deficient T cells expressing a chimeric receptor
EP2528944B1 (en) 2010-01-29 2015-08-12 MorphoSys AG Rodent combinatorial antibody libraries
ES2696548T3 (en) 2010-11-19 2019-01-16 Morphosys Ag A collection of antibody sequences and their use
US9833476B2 (en) 2011-08-31 2017-12-05 The Trustees Of Dartmouth College NKP30 receptor targeted therapeutics
CN110511278A (en) 2012-05-07 2019-11-29 达特茅斯大学理事会 Anti- B7-H6 antibody, fusion protein and its application method
CN106574270B (en) * 2014-07-03 2021-07-13 豪夫迈·罗氏有限公司 Polypeptide expression system
EA201792661A1 (en) 2015-06-01 2018-05-31 Медиджин Иммьюнотерапиз Гмбх METHOD OF OBTAINING ANTIBODIES AGAINST T-CELL RECEPTOR
CA2987857A1 (en) 2015-06-01 2016-12-08 Medigene Immunotherapies Gmbh T cell receptor library
HUE053674T2 (en) 2015-12-23 2021-07-28 Medigene Immunotherapies Gmbh Novel generation of antigen-specific tcrs
JP6818134B2 (en) 2016-09-29 2021-01-20 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ An improved way to select polypeptide-producing cells

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5874299A (en) * 1990-08-29 1999-02-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US6162963A (en) * 1990-01-12 2000-12-19 Abgenix, Inc. Generation of Xenogenetic antibodies

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6162963A (en) * 1990-01-12 2000-12-19 Abgenix, Inc. Generation of Xenogenetic antibodies
US5874299A (en) * 1990-08-29 1999-02-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HE M. ET AL.: "Selection of a human anti-progesterone antibody fragment from a transgenic mouse library by ARM ribosome display", J. IMMUNOL. METH., vol. 231, no. 1-2, 1999, pages 105 - 117, XP004187638 *
LORIMER I.A.J. ET AL.: "Recombinant immunotoxins specific for a mutant epidermal growth factor receptor: targeting with a single chain antibody variable domain isolated by phage display", PROC. NATL. ACAD. SCI. USA, vol. 93, no. 93, December 1996 (1996-12-01), pages 14815 - 14820, XP002912821 *
MCCALL A.M.: "Characterization of anti-mouse FcgammaRII single-chain Fv fragments derived fromhuman phage display libraries", IMMUNOTECHNOLOGY, vol. 4, 1998, pages 71 - 87, XP002908156 *
MEULEMANS E.V.: "Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody", J. MOL. BIOL., vol. 244, 1994, pages 353 - 360, XP002908155 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9181527B2 (en) 2009-10-29 2015-11-10 The Trustees Of Dartmouth College T cell receptor-deficient T cell compositions

Also Published As

Publication number Publication date
AU6263601A (en) 2001-12-11
IL136459A0 (en) 2001-06-14
WO2001092291A2 (en) 2001-12-06

Similar Documents

Publication Publication Date Title
WO2001092291A3 (en) Antibody and t-cell receptor libraries
EP1741780A3 (en) High affinity oligonucleotide ligands to growth factors
DE69827745D1 (en) COVALENT BINDING OF DNA TO RNA STRANDS CATALYZED BY VACCINIA TOPOISOMERASE
WO2005003291A3 (en) Bead bound combinatorial oligonucleoside phosphorothioate and phosphorodithioate aptamer libraries
WO2003046173A1 (en) siRNA EXPRESSION SYSTEM AND PROCESS FOR PRODUCING FUNCTIONAL GENE-KNOCKDOWN CELLS AND THE LIKE USING THE SAME
WO2004029220A3 (en) Synthetic genes
WO2003060084A3 (en) Methods for making polynucleotides and purifying double-stranded polynucleotides
WO2003046186A1 (en) siRNA EXPRESSION SYSTEM AND METHOD FOR PRODUCING FUNCTIONAL GENE KNOCK-DOWN CELLS USING THE SYSTEM
WO2003072753A3 (en) Multimeric binding complexes
WO2002040668A3 (en) Proteins and dna sequences underlying these proteins used for treating inflammations
EP1310790A3 (en) Multi-column affinity detection system
WO2003066810A3 (en) Recombinant bovine immunodeficiency virus based gene transfer system
NO991729D0 (en) Anti-p53 single chain antibody fragments and their use
WO2001066752A3 (en) Reproduction-specific genes
WO2002029016A3 (en) Thermostable polymerase based on thermococcus pacificus
AU5041001A (en) G protein coupled receptor
EP1816203A4 (en) An expression vector encoding coronavirus-like particles
WO2003040289A3 (en) Genes coding for dna replication proteins and for proteins related to pathogenesis
WO2003044484A3 (en) High-density cell microarrays for parallel functional determinations
WO2002046378A3 (en) Alternative pol k nucleotide and amino acid sequence and methods for using
WO2003022992A3 (en) Mammalian genes; related reagents
WO2003010309A1 (en) Oligonucleotides for detecting tubercle bacillus and method therefor
WO2003040292A3 (en) Genes encoding for membrane synthesis and membrane transport proteins
WO2003004678A3 (en) Novel receptors
WO2001046226A3 (en) Chlamydia antigens and corresponding dna fragments and uses thereof

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP