WO2002062774A1 - Thiazole derivatives for treating ppar related disorders - Google Patents

Thiazole derivatives for treating ppar related disorders

Info

Publication number
WO2002062774A1
WO2002062774A1 PCT/US2001/049230 US0149230W WO02062774A1 WO 2002062774 A1 WO2002062774 A1 WO 2002062774A1 US 0149230 W US0149230 W US 0149230W WO 02062774 A1 WO02062774 A1 WO 02062774A1
Authority
WO
WIPO (PCT)
Prior art keywords
methyl
phenoxy
thiazol
propionic acid
phenyl
Prior art date
Application number
PCT/US2001/049230
Other languages
French (fr)
Inventor
Rodolfo Cadilla
Romain Luc Marie Gosmini
Millard Hurst Lambert, Iii
Michael Lawrence Sierra
Original Assignee
Glaxo Group Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CA002432188A priority Critical patent/CA2432188A1/en
Priority to NZ526543A priority patent/NZ526543A/en
Priority to JP2002562729A priority patent/JP4209681B2/en
Priority to AU2002246713A priority patent/AU2002246713B2/en
Priority to EP01994305A priority patent/EP1343773B1/en
Priority to DK01994305T priority patent/DK1343773T3/en
Priority to PL01365797A priority patent/PL365797A1/en
Priority to MXPA03005699A priority patent/MXPA03005699A/en
Priority to HU0400837A priority patent/HUP0400837A3/en
Priority to SI200130672T priority patent/SI1343773T1/en
Application filed by Glaxo Group Limited filed Critical Glaxo Group Limited
Priority to IL15643601A priority patent/IL156436A0/en
Priority to BR0116370-1A priority patent/BR0116370A/en
Priority to US10/451,313 priority patent/US7105551B2/en
Priority to DE60124302T priority patent/DE60124302T2/en
Priority to KR1020037008392A priority patent/KR100815022B1/en
Publication of WO2002062774A1 publication Critical patent/WO2002062774A1/en
Priority to IL156436A priority patent/IL156436A/en
Priority to NO20032801A priority patent/NO20032801L/en
Priority to HK04101319A priority patent/HK1059925A1/en
Priority to US11/465,135 priority patent/US7439259B2/en
Priority to CY20071100032T priority patent/CY1105915T1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
    • C07D277/02—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings
    • C07D277/20—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D277/22—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
    • C07D277/24—Radicals substituted by oxygen atoms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/04—Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
    • C07D277/02—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings
    • C07D277/20—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D277/22—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
    • C07D277/26—Radicals substituted by sulfur atoms

Definitions

  • the present invention relates to certain novel compounds.
  • the present invention relates to compounds that activate human peroxisome proliferator activated receptors ("hPPARs").
  • the present invention also relates to method for preparing the compounds, their use in medicine, pharmaceutical compositions containing them and methods for the prevention or treatment of PPAR mediated diseases or conditions.
  • HMG CoA reductase inhibitors are useful for treating conditions characterized by high LDL-c levels. It has been shown that lowering LDL-c is not sufficient for reducing the risk of cardiovascular disease in some patients, particularly those with normal LDL-c levels. This population pool is identified by the independent risk factor of low HDL-c.
  • the increased risk of cardiovascular disease associated with low HDL-c levels has not yet been successfully addressed by drug therapy (i.e. currently there are no drugs on the market that are useful for raising HDL-c). (Bisgaier, C. L.; Pape, M. E. Curr. Pharm. Des. 1998, 4, 53-70).
  • Syndrome X (including metabolic syndrome) is loosely defined as a collection of abnormalities including hyperinsulemia, obesity, elevated levels of triglycerides, uric acid, fibrinogen, small dense LDL particles, and plasminogen activator inhibitor 1 (PAI-1), and decreased levels of HDL : c.
  • abnormalities including hyperinsulemia, obesity, elevated levels of triglycerides, uric acid, fibrinogen, small dense LDL particles, and plasminogen activator inhibitor 1 (PAI-1), and decreased levels of HDL : c.
  • NIDD insulin resistance
  • IGT impaired glucose tolerance
  • PPARs Peroxisome Proliferator Activated Receptors
  • PPARs are orphan receptors belonging to the steroid/retinoid receptor superfamily of ligand-activated transcription factors. See, for example Willson T.M. and Wahli, W., Curr. Opin. Chem. Biol., 1 , pp235-241 (1997) and Willson T.M. et. al., J. Med. Chem., 43, p527-549 (2000).
  • PPAR-alpha Three mammalian Peroxisome Proliferator-Activated Receptors have been isolated and termed PPAR-alpha, PPAR-gamma, and PPAR-delta (also known as NUC1 or PPAR-beta). These PPARs regulate expression of target genes by binding to DNA sequence elements, termed PPAR response elements (PPRE).
  • PPRE PPAR response elements
  • thiazolidinediones are potent and selective activators of PPAR-gamma and bind directly to the PPAR-gamma receptor (J. M. Lehmann et. al., J. Biol. Chem. 12953-12956, 270 (1995)), providing evidence that PPAR-gamma is a possible target for the therapeutic actions of the thiazolidinediones.
  • Activators of the nuclear receptor PPAR ⁇ for example troglitazone, have been shown in the clinic to enhance insulin-action, reduce serum glucose and have small but significant effects on reducing serum triglyceride levels in patients with Type 2 diabetes.
  • VLDL very low density lipoproteins
  • LPL lipoprotein lipase
  • Patents 5,847,008 Doebber et al. and 5,859,051 (Adams et al.) and PCT publications WO 97/28149 (Leibowitz et al.) and WO99/04815 (Shimokawa et al.).
  • PPAR ⁇ activation does not appear to modulate glucose or triglyceride levels.
  • the invention provides a compound of formula 1 and pharmaceutically acceptable salts and solvates and hydrolysable esters thereof.
  • R 1 and R 2 are independently H or C ⁇ alkyl, or R 1 and R 2 may together with the carbon atom to which they are bonded form a 3-6 membered cycloalkyl ring, and at least one of R 1 and
  • R 2 must be other than H
  • X 2 is O, S, or (CR 10 R 11 ) n where n is 1 or 2 and R 10 and R 1 are independently H, fluorine, or C ⁇ alkyl;
  • R 3 , R 4 , and R 5 are independently H, C ⁇ alkyl, OCH 3 , CF 3 , allyl, or halogen.
  • one of Y and Z is N, the other is S or O;
  • R 6 and R 7 are independently H, phenyl, benzyl, fluorine, OH, C ⁇ alkyl, allyl, or R 6 and R 7 may together with the carbon atom to which they are bonded form a carbonyl group;
  • R 9 is H, CF 3 , or C ⁇ alkyl;
  • Each R 8 is independently CF 3 , C ⁇ alkyl, OCH 3 , or halogen; y is 0, 1 , 2, 3, 4, or 5.
  • the present invention discloses a method for prevention or treatment of a disease or condition mediated by one or more human PPAR alpha, gamma or delta (“hPPARs") comprising administration of a therapeutically effective amount of a compound of this invention.
  • hPPAR mediated diseases or conditions include dyslipidemia including associated diabetic dyslipidemia and mixed dyslipidemia, syndrome X (as defined in this application this embraces metabolic syndrome), heart failure, hypercholesteremia, cardiovascular disease including atherosclerosis, arteriosclerosis, and hypertriglyceridemia, type II diabetes mellitus, type I diabetes, insulin resistance, hyperlipidemia, inflammation, epithelial hyperproliferative diseases including eczema and psoriasis and conditions associated with the lung and gut and regulation of appetite and food intake in subjects suffering from disorders such as obesity, anorexia bulimia, and anorexia nervosa.
  • disorders such as obesity, anorexia bulimia, and anorexia nervosa.
  • the compounds of this invention are useful in the treatment and prevention of diabetes and cardiovascular diseases and conditions including atherosclerosis, arteriosclerosis, hypertriglyceridemia, and mixed dyslipidaemia.
  • the present invention provides pharmaceutical compositions comprising a compound of the invention, preferably in association with a pharmaceutically acceptable diluent or carrier.
  • the present invention provides a compound of the invention for use in therapy, and in particular, in human medicine.
  • the present invention provides the use of a compound of the invention for the manufacture of a medicament for the treatment of a hPPAR mediated disease or condition.
  • a compound of the invention means a compound of formula (I) or a pharmaceutically acceptable salt, or solvate, or hydrolysable ester thereof.
  • hydrolyzable esters are included in the scope of this invention, the acids are preferred because the data suggests that while the esters are useful compounds, it may actually be the acids to which they hydrolyse that are the active compounds.
  • Esters that hydrolyse readily can produce the carboxylic acid in the assay conditions or in vivo. Generally the carboxylic acid is active in both the binding and transient transfection assays, while the ester does not usually bind well but is active in the transient transfection assay presumably due to hydrolysis.
  • Preferred hydrolysable esters are C ⁇ alkyl esters wherein the alkyl group may be straight chain or branched chain. Methyl or ethyl esters are more preferred.
  • at least one of R 1 and R 2 is CH 3 . More preferably R 1 and R 2 are both CH 3 ;
  • X 2 is O, S, or C(R 10 R 11 ). Most preferably X 2 is O or S;
  • R 3 is CH 3 ;
  • R 4 and R 5 are independently H or methyl
  • Z is N;
  • Y is S.
  • R 10 and R 11 are H;
  • R 9 is CH 3 .
  • R 6 is H, CH 3, CH 2 CH 3 or allyl. Most preferably R 6 is H, CH 3 , or CH 2 CH 3 .
  • R 7 is H.
  • each R 8 is independently F or CF 3 .
  • y is 1 or 2.
  • y is 2, preferably one of the substituents is halogen and the other is CF 3 .
  • y is 1 , preferably the substituent is in the para position on the ring.
  • preferred compounds of this invention include those in which several or each variable in Formula (I) is selected from the preferred, more preferred, or most preferred groups for each variable. Therefore, this invention is intended to include all combinations of preferred and most preferred groups.
  • Preferred compounds of formula 1 include:
  • Most preferred compounds of formula 1 include: 2- ⁇ 4-[( ⁇ 2-[2-fluoro-4-(trifluoromethyl)phenyl]-4-methyl-1 ,3-thiazol-5-yl ⁇ methyl)sulfanyl]-2- methylphenoxy ⁇ -2-methylpropanoic acid
  • the present invention includes all possible stereoisomers and geometric isomers of formula (I) and includes not only racemic compounds but this invention is also intended to cover each of these isomers in their racemic, enriched, or purified forms.
  • a compound of formula (I) When a compound of formula (I) is desired as a single enantiomer, it may be obtained either by resolution of the final product or by stereospecific synthesis using an optically active catalyst or a catalytic system with optically active ligands or isomerically pure starting material or any convenient intermediate. Resolution of the final product, an intermediate or a starting material may be effected by any suitable method known in the art. See, for example, Stereochemistry of Carbon Compounds by E.
  • the present invention is intended to include all tautomeric forms of the compounds.
  • the carbon atom to which R 6 and R 7 are bonded is chiral.
  • the activities at the various PPAR receptors varies between the S and R isomers. Which of these isomers is preferred depends on the particular desired utility of the compound. In other words, even with the same compound, it is possible that the S isomer will be preferred for some uses, while the R isomer will be preferred for others.
  • the hPPAR agonists of formula (I) may be agonists of only one type ("selective agonists"), agonists for two PPAR subtypes ("dual agonists"), or agonists for all three subtypes ("pan agonists”).
  • agonist or “activating compound”, or “activator”, or the like, is meant those compounds which have a pKi of at least 6.0 preferably at least 7.0 to the relevant PPAR, for example hPPAR ⁇ , in the binding assay described below, and which achieve at least 50% activation of the relevant PPAR relative to the appropriate indicated positive control in the transfection assay described below at concentrations of 10 ⁇ 5 M or less.
  • the agonists of this invention achieve 50% activation of at least one human PPAR in the relevant transfection assay at concentrations of 10 "6 M or less.
  • the compounds of formula (I) are hPPAR agonists. More preferably the compounds are hPPAR ⁇ agonists. Most preferably they are dual agonists of hPPAR ⁇ and hPPAR ⁇ , or pan agonists.
  • the compounds of the present invention may also be utilised in the form of a pharmaceutically acceptable salt or solvate thereof.
  • physiologically acceptable salts of the compounds of formula (I) include conventional salts formed from pharmaceutically acceptable inorganic or organic acids or bases as well as quaternary ammonium acid addition salts.
  • suitable acid salts include hydrochloric, hydrobromic, sulfuric, phosphoric, nitric, perchloric, fumaric, acetic, propionic, succinic, glycolic, formic, lactic, maleic, tartaric, citric, palmoic, malonic, hydroxymaleic, phenylacetic, glutamic, benzoic, salicylic, fumaric, toluenesulfonic, methanesulfonic, naphthalene- 2-sulfonic, benzenesulfonic hydroxynaphthoic, hydroiodic, malic, steroic, tannic and the like.
  • acids such as oxalic, while not in themselves pharmaceutically acceptable, may be useful in the preparation of salts useful as intermediates in obtaining the compounds of the invention and their pharmaceutically acceptable salts.
  • suitable basic salts include sodium, lithium, potassium, magnesium, aluminium, calcium, zinc, N,N'-dibenzylethylenediamine, chloroprocaine, choline, diethanolamine, ethylenediamine, N-methylglucamine and procaine salts.
  • compositions are conveniently administered in the form of pharmaceutical compositions.
  • Such compositions may conveniently be presented for use in conventional manner in admixture with one or more physiologically acceptable carriers or excipients.
  • the active ingredient may be therapeutically administered as the raw chemical
  • the carrier(s) must be "acceptable” in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.
  • the present invention further provides for a pharmaceutical formulation comprising a compound of formula (I) or a pharmaceutically acceptable salt or solvate thereof together with one or more pharmaceutically acceptable carriers therefore and, optionally, other therapeutic and/or prophylactic ingredients.
  • the formulations include those suitable for oral, parenteral (including subcutaneous e.g. by injection or by depot tablet, intradermal, intrathecal, intramuscular e.g. by depot and intravenous), rectal and topical (including dermal, buccal and sublingual) administration although the most suitable route may depend upon for example the condition and disorder of the recipient.
  • the formulations may conveniently be presented in unit dosage form and may be prepared by any of the methods well known in the art of pharmacy. All methods include the step of bringing into association the compounds ("active ingredient") with the carrier, which constitutes one or more accessory ingredients. In general the formulations are prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers or finely divided solid carriers or both and then, if necessary, shaping the product into the desired formulation.
  • Formulations suitable for oral administration may be presented as discrete units such as capsules, cachets or tablets (e.g. chewable tablets in particular for paediatric administration) each containing a predetermined amount of the active ingredient; as a powder or granules; as a solution or a suspension in an aqueous liquid or a non-aqueous liquid; or as an oil-in-water liquid emulsion or a water-in-oil liquid emulsion.
  • the active ingredient may also be presented as a bolus, electuary or paste.
  • a tablet may be made by compression or moulding, optionally with one or more accessory ingredients.
  • Compressed tablets may be prepared by compressing in a suitable machine the active ingredient in a free-flowing form such as a powder or granules, optionally mixed with a other conventional excipients such as binding agents, (for example, syrup, acacia, gelatin, sorbitol, tragacanth, mucilage of starch or polyvinylpyrrolidone), fillers (for example, lactose, sugar, microcrystalline cellulose, maize-starch, calcium phosphate or sorbitol), lubricants (for example, magnesium stearate, stearic acid, talc, polyethylene glycol or silica), disintegrants (for example, potato starch or sodium starch glycollate) or wetting agents, such as sodium lauryl sulfate.
  • binding agents for example, syrup, acacia, gelatin, sorbitol, tragacanth, m
  • Moulded tablets may be made by moulding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent.
  • the tablets may optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active ingredient therein.
  • the tablets may be coated according to methods well-known in the art.
  • the compounds of the present invention may be incorporated into oral liquid preparations such as aqueous or oily suspensions, solutions, emulsions, syrups or elixirs, for example.
  • formulations containing these compounds may be presented as a dry product for constitution with water or other suitable vehicle before use.
  • Such liquid preparations may contain conventional additives such as suspending agents such as sorbitol syrup, methyl cellulose, glucose/sugar syrup, gelatin, hydroxyethylcellulose, carboxymethyl cellulose, aluminum stearate gel or hydrogenated edible fats; emulsifying agents such as lecithin, sorbitan mono- oleate or acacia; non-aqueous vehicles (which may include edible oils) such as almond oil, fractionated coconut oil, oily esters, propylene glycol or ethyl alcohol; and preservatives such as methyl or propyl p-hydroxybenzoates or sorbic acid.
  • Such preparations may also be formulated as suppositories, e.g., containing conventional suppository bases such as cocoa butter or other glycerides.
  • Formulations for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non- aqueous sterile suspensions which may include suspending agents and thickening agents.
  • the formulations may be presented in unit-dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in a freeze-dried (lyophilised) condition requiring only the addition of a sterile liquid carrier, for example, water-for-injection, immediately prior to use.
  • Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets of the kind previously described.
  • Formulations for rectal administration may be presented as a suppository with the usual carriers such as cocoa butter, hard fat or polyethylene glycol.
  • Formulations for topical administration in the mouth include lozenges comprising the active ingredient in a flavoured basis such as sucrose and acacia or tragacanth, and pastilles comprising the active ingredient in a basis such as gelatin and glycerin or sucrose and acacia.
  • the compounds may also be formulated as depot preparations. Such long acting formulations may be administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection.
  • the compounds may be formulated with suitable polymeric or hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt.
  • the formulations may include other agents conventional in the art having regard to the type of formulation in question, for example those suitable for oral administration may include flavouring agents.
  • reference herein to treatment extends to prophylaxis as well as the treatment of established diseases or symptoms.
  • the amount of a compound of the invention required for use in treatment will vary with the nature of the condition being treated and the age and the condition of the patient and will be ultimately at the discretion of the attendant physician or veterinarian. In general, however, doses employed for adult human treatment will typically be in the range of 0.02-5000 mg per day, preferably 1-1500 mg per day.
  • the desired dose may conveniently be presented in a single dose or as divided doses administered at appropriate intervals, for example as two, three, four or more sub-doses per day.
  • the formulations according to the invention may contain between 0.1-99% of the active ingredient, conveniently from 30-95% for tablets and capsules and 3-50% for liquid preparations.
  • the compound of formula (I) for use in the instant invention may be used in combination with other therapeutic agents for example, statins and/or other lipid lowering drugs for example
  • the compounds of the invention may also be used in combination with antidiabetic agents, e.g. metformin, sulfonylureas and/or PPAR gamma, PPAR alpha or PPAR alpha/gamma agonists (for example thiazolidinediones such as e.g. Pioglitazone and Rosiglitazone).
  • antidiabetic agents e.g. metformin, sulfonylureas and/or PPAR gamma, PPAR alpha or PPAR alpha/gamma agonists
  • thiazolidinediones for example thiazolidinediones such as e.g. Pioglitazone and Rosiglitazone
  • antihypertensive agents such as angistensin antagonists e.g. telmisartan, calcium channel antagonists e.g. lacidipine and ACE inhibitors e.g. en
  • the compounds of formula (I) When used in combination with other therapeutic agents, the compounds may be administered either sequentially or simultaneously by any convenient route.
  • compositions comprising a combination as defined above optimally together with a pharmaceutically acceptable carrier or excipient comprise a further aspect of the invention.
  • the individual components of such combinations may be administered either sequentially or simultaneously in separate or combined pharmaceutical formulations.
  • the two compounds When combined in the same formulation it will be appreciated that the two compounds must be stable and compatible with each other and the other components of the formulation and may be formulated for administration. When formulated separately they may be provided in any convenient formulation, conveniently in such a manner as are known for such compounds in the art.
  • each compound of formula (I) When a compound of formula (I) is used in combination with a second therapeutic agent active against the same hPPAR mediated disease, the dose of each compound may differ from that when the compound is used alone. Appropriate doses will be readily appreciated by those skilled in the art.
  • Compounds of this invention may be conveniently prepared by a general process wherein a moiety like A is coupled to an alcohol (B and D) using the Mitsunobu protocol (O. Mitsunobu, 1981 Synthesis, p 1 ) or by alkylation of A using a suitable non nucleophiiic base such as K 2 C0 3 , Cs 2 C0 3 or NaH, with an alkyl halide (C, E and F).
  • this synthesis is preferably carried out with the acid group protected by R.
  • R is 1-6 alkyl which can be hydrolysed to give an acid of Formula (I), or if readily hydrolyzable, the resulting ester can be administered.
  • Y is S
  • Z is N
  • R 1 , R 2 , R 3 and R 6 are CH 3
  • R 7 is H
  • X 2 is 0
  • R 8 is para-CF 3 :
  • Compounds used as starting materials are either commercially available compounds or known compounds.
  • reaction mixture was diluted with CH 2 CI 2 (120mL) and washed with NaHC0 3 (sat.) (2 x 240mL) and water (2 x 240mL), dried, filtered and evaporated to afford the title compound (8.0g, 27 mmol, 90%) as a yellow solid.
  • Example 7 2-methyl-2- (2-methyl-4- ⁇ 1-[4-methyl-2- (3-fluoro-4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy ⁇ -phenoxy)-propionic acid
  • Example 8 The same saponification protocol as for example 3 was applied usingl .6g of Example 8 (1.6g, 3.15 mmol).
  • example 26 To a solution of example 26 (1.1g, 2.17 mmol) in THF / ethanol (1/1 ) was added 1 N NaOH (10 mmol, 4.6 eq). The resulting mixture was stirred at 80°C for 1.5 hour, and after cooling at room temperature 1 N HCI (10 mmol, 4.6 eq) was added. The resulting mixture was concentrated in vacuo, and the residue was sonicated in water / EtOH. The resulting solid was filtered off and washed with water , diluted HCI, and water to give the title compound (680mg, 1.38 mmol) in a
  • example 32 (1g, 1.87 mmol) in THF / ethanol (1/1 ) was added dropwise 0.2N NaOH (5.6 mmol, 3 eq). The resulting mixture was stirred at 50 °C for 3 hours, and after cooling at 0°C 0.2N HCI (5.6 mmol, 3eq) was added. The resulting mixture was concentrated in vacuo, then the residue was taken up in water and extracted with ethyl acetate. The organic phase was evaporated off and the residue was purified by flash chromatography using CH 2 CI 2 / MeOH (97/3) as eluent.
  • Example 37 2-methyl- ⁇ 2-methyl-2- (2-methyl-4- ⁇ 1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl1- propoxy ⁇ -phenoxy) ⁇ -propionic acid
  • example 36 450 mg, 0.84 mmol
  • THF/Ethanol 5/20mL
  • 1 N NaOH 5mmol, 5mL
  • the resulting mixture was stirred at 50 °C for 2 hours, and after cooling at room temperature 1 N HCI (5 mmol, 5mL) was added.
  • the resulting mixture was concentrated in vacuo and the residue was flash chromatographed CH 2 CI 2 / EtOAc (95:5) to give the title compound (290 mg, 0.57 mmol) in a 68 % yield as an amorphous yellow powder.
  • Example 39 2-methyl-2- (2-methyl-4- ⁇ 1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl1 pentyloxy ⁇ - phenoxy)-propionic acid
  • example 38 To a solution of example 38 (90 mg, 0.164 mmol) in THF/Ethanol (1/1) was added 1 N NaOH (5 mmol, 5mL). The resulting mixture was stirred at 80 °C for 1.5 hour, and after cooling at room temperature 1 N HCI (5 mmol, 5mL) was added. The resulting mixture was concentrated in vacuo, then the residue was flash chromatographed eluting with CH 2 CI 2 / EtOAc (90:10) to give the title compound (20 mg, 0.038 mmol) in a 23% yield as a colorless oil.
  • example 42 To a solution of example 42 (740mg, 1.3 mmol) in THF / ethanol (1/1) was added dropwise 0.2N NaOH (6.5 mmol, 5 eq). The resulting mixture was stirred at 50°C for 4 hours, and after cooling at 0°C 0.2N HCI (6.5 mmol, 5 eq) was added. The resulting mixture was concentrated in vacuo, then the residue was taken up in water and extracted with ethyl acetate. The organic phase was evaporated off and the residue was purified by flash chromatography using CH 2 CI 2 / ethyl acetate (80/20) as eluent.
  • intermediate 16 (287mg, 1 mmol) and Znl 2 (160mg, O. ⁇ mmol) in 10mL CH 2 CI 2 was added intermediate thiophenol 44 (305mg, 1.2mmol) in solution in 10 mL of CH 2 CI 2 .
  • Example 52 5 2-methyl-2-(2-methyl-4- ⁇ 1-methyl-1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]- ethylsulfanyl ⁇ -phenoxy)-propionic acid ethyl ester
  • This compound was used as a PPARdelta reference in the transfection assays described below and was prepared according to the method reported in WO200100603-A1
  • This compound was used as a PPAR alpha reference in the transfection assay described below and was prepared according to method reported in WO200140207-A1
  • Binding Assay Compounds were tested for their ability to bind to hPPAR gamma hPPARalpha. or
  • PPARdelta using a Scintillation Proximity Assay (SPA).
  • SPA Scintillation Proximity Assay
  • the PPAR ligand binding domain (LBD) was expressed in E. coli as polyHis tagged fusion proteins and purified. The LBD was then labelled with biotin and immobilised on streptavidin-modified scintillation proximity beads.
  • An antidiabetic thiazolidinedione is a high affinity ligand for peroxisome proliferator-activated receptor gamma (PPARgammaJ, J. Biol. Chem., 270, 12953-6 (1995).
  • the ligand binding domains for murine and human PPAR alpha, PPAR gamma, and PPAR delta were each fused to the yeast transcription factor GAL4 DNA binding domain.
  • CV-1 cells were transiently transfected with expression vectors for the respective PPAR chimera along with a reporter construct containing five copies of the GAL4 DNA binding site driving expression of secreted placental alkaline phosphatase (SPAP) and beta-galactosidase.
  • SPAP secreted placental alkaline phosphatase
  • the positive control in the hPPAR alpha assays was 2-methyl-2-[4- ⁇ [(4-methyl-2-[4-trifluoromethylphenyl]-thiazol-5-yl-carbonyl)amino]methyl ⁇ - phenoxy]propionic acid.
  • the positive control for PPAR delta assays was 2- ⁇ 2-methyl-4-[( ⁇ 4- methyl-2- ⁇ trifluoromethyl)phenyl]-1 ,3-thiazol-5-yl ⁇ methyl)sulfanyl]phenoxy ⁇ acetic acid.

Abstract

The present invention provides compounds of formula (I). These compounds are used for the treatment of PPAR related diseases.

Description

THIAZOLE DERIVATIVES FOR TREATING PPAR RELATED DISORDERS
The present invention relates to certain novel compounds. In particular, the present invention relates to compounds that activate human peroxisome proliferator activated receptors ("hPPARs"). The present invention also relates to method for preparing the compounds, their use in medicine, pharmaceutical compositions containing them and methods for the prevention or treatment of PPAR mediated diseases or conditions.
Several independent risk factors have been associated with cardiovascular disease. These include hypertension, increased fibrinogen levels, high levels of triglycerides, elevated LDL cholesterol, elevated total cholesterol, and low levels of HDL cholesterol. HMG CoA reductase inhibitors ("statins") are useful for treating conditions characterized by high LDL-c levels. It has been shown that lowering LDL-c is not sufficient for reducing the risk of cardiovascular disease in some patients, particularly those with normal LDL-c levels. This population pool is identified by the independent risk factor of low HDL-c. The increased risk of cardiovascular disease associated with low HDL-c levels has not yet been successfully addressed by drug therapy (i.e. currently there are no drugs on the market that are useful for raising HDL-c). (Bisgaier, C. L.; Pape, M. E. Curr. Pharm. Des. 1998, 4, 53-70).
Syndrome X (including metabolic syndrome) is loosely defined as a collection of abnormalities including hyperinsulemia, obesity, elevated levels of triglycerides, uric acid, fibrinogen, small dense LDL particles, and plasminogen activator inhibitor 1 (PAI-1), and decreased levels of HDL:c.
NIDD is described as insulin resistance, which in turn causes anomalous glucose output and a decrease in glucose uptake, by skeletal muscle. These factors eventually lead to impaired glucose tolerance (IGT) and hyperinsulinemia. Peroxisome Proliferator Activated Receptors (PPARs) are orphan receptors belonging to the steroid/retinoid receptor superfamily of ligand-activated transcription factors. See, for example Willson T.M. and Wahli, W., Curr. Opin. Chem. Biol., 1 , pp235-241 (1997) and Willson T.M. et. al., J. Med. Chem., 43, p527-549 (2000). The binding of agonist ligands to the receptor results in changes in the expression level of MRNA's enclded by PPAR target genes. Three mammalian Peroxisome Proliferator-Activated Receptors have been isolated and termed PPAR-alpha, PPAR-gamma, and PPAR-delta (also known as NUC1 or PPAR-beta). These PPARs regulate expression of target genes by binding to DNA sequence elements, termed PPAR response elements (PPRE). To date, PPRE's have been identified in the enhancers of a number of genes encoding proteins that regulate lipid metabolism suggesting that PPARs play a pivotal role in the adipogenic signalling cascade and lipid homeostasis (H. Keller and W. Wahli,
Trends Endocrinol. Metab 291-296, 4 (1993)). It has now been reported that thiazolidinediones are potent and selective activators of PPAR-gamma and bind directly to the PPAR-gamma receptor (J. M. Lehmann et. al., J. Biol. Chem. 12953-12956, 270 (1995)), providing evidence that PPAR-gamma is a possible target for the therapeutic actions of the thiazolidinediones. Activators of the nuclear receptor PPARγ, for example troglitazone, have been shown in the clinic to enhance insulin-action, reduce serum glucose and have small but significant effects on reducing serum triglyceride levels in patients with Type 2 diabetes. See, for example, D. E. Kelly et al., Curr. Opin. Endocrinol. Diabetes, 90-96, 5 (2), (1998); M. D. Johnson et al., Ann. Pharmacother., 337-348, 32 (3), (1997); and M. Leutenegger et al., Curr. Ther. Res., 403-416, 58 (7), (1997).
The mechanism for this triglyceride lowering effect appears to be predominantly increased clearance of very low density lipoproteins (VLDL) through induction of lipoprotein lipase (LPL) gene expression. See, for example, B. Staels et al., Aderioscler. Thromb., Vase. Biol., 1756- 1764, 17 (9), (1997). Fibrates are a class of drugs which may lower serum triglycerides 20-50%, lower LDLc 10-
15%, shift the LDL particle size from the more atherogenic small dense to normal dense LDL, and increase HDLc 10-15%. Experimental evidence indicates that the effects of fibrates on serum lipids are mediated through activation of PPARα. See, for example, B. Staels et al., Curr. Pharm. Des., 1-14, 3 (1 ), (1997). Activation of PPARα results in transcription of enzymes that increase fatty acid catabolism and decrease de-novo fatty acid synthesis in the liver resulting in decreased triglyceride synthesis and VLDL production/secretion. In addition, PPARα activation decreases production of apoC-lll. Reduction in apoC-lll, an inhibitor of LPL activity, increases clearance of VLDL. See, for example, J. Auwerx et al., Atherosclerosis, (Shannon, lrel.)t S29-S37, 124 (Suppl), (1996). Certain compounds that activate or otherwise interact with one or more of the PPARs have been implicated in the regulation of triglyceride and cholesterol levels in animal models. See, for example, U.S. Patents 5,847,008 (Doebber et al.) and 5,859,051 (Adams et al.) and PCT publications WO 97/28149 (Leibowitz et al.) and WO99/04815 (Shimokawa et al.). In a recent report (Berger et al., J. Biol. Chem. 1999), vol. 274, pp. 6718-6725) it was stated that PPARδ activation does not appear to modulate glucose or triglyceride levels.
Accordingly the invention provides a compound of formula 1 and pharmaceutically acceptable salts and solvates and hydrolysable esters thereof.
Figure imgf000004_0001
(1)
wherein:
R1 and R2 are independently H or C^ alkyl, or R1 and R2 may together with the carbon atom to which they are bonded form a 3-6 membered cycloalkyl ring, and at least one of R1 and
R2 must be other than H;
X2 is O, S, or (CR10R11)n where n is 1 or 2 and R10 and R 1 are independently H, fluorine, or C^alkyl;
R3, R4, and R5 are independently H, C^alkyl, OCH3, CF3, allyl, or halogen. one of Y and Z is N, the other is S or O;
R6 and R7 are independently H, phenyl, benzyl, fluorine, OH, C^ alkyl, allyl, or R6 and R7 may together with the carbon atom to which they are bonded form a carbonyl group; R9 is H, CF3, or C^alkyl;
Each R8 is independently CF3, C^alkyl, OCH3, or halogen; y is 0, 1 , 2, 3, 4, or 5.
In another aspect, the present invention discloses a method for prevention or treatment of a disease or condition mediated by one or more human PPAR alpha, gamma or delta ("hPPARs") comprising administration of a therapeutically effective amount of a compound of this invention. hPPAR mediated diseases or conditions include dyslipidemia including associated diabetic dyslipidemia and mixed dyslipidemia, syndrome X (as defined in this application this embraces metabolic syndrome), heart failure, hypercholesteremia, cardiovascular disease including atherosclerosis, arteriosclerosis, and hypertriglyceridemia, type II diabetes mellitus, type I diabetes, insulin resistance, hyperlipidemia, inflammation, epithelial hyperproliferative diseases including eczema and psoriasis and conditions associated with the lung and gut and regulation of appetite and food intake in subjects suffering from disorders such as obesity, anorexia bulimia, and anorexia nervosa. In particular, the compounds of this invention are useful in the treatment and prevention of diabetes and cardiovascular diseases and conditions including atherosclerosis, arteriosclerosis, hypertriglyceridemia, and mixed dyslipidaemia. In another aspect, the present invention provides pharmaceutical compositions comprising a compound of the invention, preferably in association with a pharmaceutically acceptable diluent or carrier. In another aspect, the present invention provides a compound of the invention for use in therapy, and in particular, in human medicine.
In another aspect, the present invention provides the use of a compound of the invention for the manufacture of a medicament for the treatment of a hPPAR mediated disease or condition. As used herein, "a compound of the invention" means a compound of formula (I) or a pharmaceutically acceptable salt, or solvate, or hydrolysable ester thereof.
While hydrolyzable esters are included in the scope of this invention, the acids are preferred because the data suggests that while the esters are useful compounds, it may actually be the acids to which they hydrolyse that are the active compounds. Esters that hydrolyse readily can produce the carboxylic acid in the assay conditions or in vivo. Generally the carboxylic acid is active in both the binding and transient transfection assays, while the ester does not usually bind well but is active in the transient transfection assay presumably due to hydrolysis. Preferred hydrolysable esters are C^ alkyl esters wherein the alkyl group may be straight chain or branched chain. Methyl or ethyl esters are more preferred. Preferably at least one of R1 and R2 is CH3. More preferably R1 and R2 are both CH3;
Preferably X2 is O, S, or C(R10R11). Most preferably X2 is O or S;
Preferably R3 is CH3;
Preferably R4 and R5 are independently H or methyl;
Preferably Z is N; Preferably Y is S.
Preferably R10 and R11 are H;
Preferably R9 is CH3.
Preferably R6 is H, CH3, CH2CH3 or allyl. Most preferably R6 is H, CH3, or CH2CH3.
Preferably R7 is H. Preferably each R8 is independently F or CF3.
Preferably y is 1 or 2. When y is 2, preferably one of the substituents is halogen and the other is CF3. When y is 1 , preferably the substituent is in the para position on the ring.
While the preferred groups for each variable have generally been listed above separately for each variable, preferred compounds of this invention include those in which several or each variable in Formula (I) is selected from the preferred, more preferred, or most preferred groups for each variable. Therefore, this invention is intended to include all combinations of preferred and most preferred groups.
Preferred compounds of formula 1 include:
2-{4-[({2-[2-fluoro-4-(trifluoromethyl)phenyl]-4-methyl-1 ,3-thiazol-5-yl}methyl)sulfanyI]-2- methylphenoxy}-2-methyIpropanoic acid
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyIphenyl)thiazol-5- yl]ethoxy}phenoxy)propionic acid ethyl ester 2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)thiazol-5- yl]propoxy}phenoxy)propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)thiazol-5-yl]but-3- enyloxy}phenoxy)propionic acid ethyl ester 2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)-thiazol-5- yl]phenylmethoxy}phenoxy)propionic acid ethyl ester
(2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}-phenoxy)-acetic acid ethyl ester
2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (3-Fluoro-4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}-phenoxy)-propionic acid ethyl ester
(S)-2-methyl-2- (2-methyl-4- {1 -[4-methyI-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}- phenoxy)-propionic acid ethyl ester
(R)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}- phenoxy)-propionic acid ethyl ester 2-(4-{1-[2-(4-chloro-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl-phenoxy)-2-methyl- propionic acid ethyl ester
2-(4-{1-[2-(3,4-dichloro-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl-phenoxy)-2-methyl- propionic acid ethyl ester
2-(4-{1-[2-(4-ethyl-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl-phenoxy)-2-methyl- propionic acid ethyl ester
2-(4-{1-[2-(2-fluoro-4-trifluoromethyl-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl- phenoxy)-2-methyl-propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethoxy}-phenoxy)- propionic acid ethyl ester 2-methyl-2-(2-methyl-4-{1 -methyl-1 -[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]- ethoxy}-phenoxy)-propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-methyl-1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethoxy}- phenoxy)-propionic acid ethyl ester
(R)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] propoxy}- phenoxy)-propionic acid ethyl ester
(S)-2-methyl-2- (2-methyl-4- {1 -[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] propoxy}- phenoxy)-propionic acid ethyl ester
2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] butoxy}- phenoxy)-propionic acid ethyl ester 2-methyl-{2-methyl-2- (2-methyl-4- {1 -[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl]- propoxy}-phenoxy)}-propionic acid ethyl ester 2-rnethyl-2- (2~methyl-4- {1-[4-methyl-2- (4-trifluoromethyI-phenyl)-thiazol-5-yl] pentyloxy}- phenoxy)-propionic acid ethyl ester
2-(4-{cyclopentyl-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-methoxy}-2-methyl- phenoxy)-2-methyl-propionic acid ethyl ester 2-methyl-2-(2-methyl-4-[4-methyl-2-(4-trifluoromethylphenyl)thiazol-5-yl- methoxy]phenoxy)propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-2-phenyl- ethoxy}-phenoxy)-propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyI)-thiazol-5-yl]-ethylsulfanyl}- phenoxy)-propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethylsulfanyl}-phenoxy)- propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-methyl-1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]- ethylsulfanyl}-phenoxy)-propionic acid ethyl ester 2-methyl-2-(2-methyl-4-{1-methyl-1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethylsulfanyl}- phenoxy)-propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]- propylsulfanyl}-phenoxy)-propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)-thiazol-5- yl]phenylmethoxy}phenoxy)propionic acid
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyI)thiazol-5- yl]ethoxy}phenoxy)propionic acid
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)thiazol-5- yl]propoxy}phenoxy)propionic acid 2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)-thiazoi-5-yl]but-3- enyloxy}phenoxy)propionic acid
(2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyl)-thiazoI-5-yl] ethoxy}-phenoxy)-acetic acid
2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (3-Fluoro-4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}-phenoxy)-propionic acid
(S)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}- phenoxy)-propionic acid
(R)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}- phenoxy)-propionic acid 2-(4-{1-[2-(4-chloro-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyI-phenoxy)-2-methyl- propionic acid 2-(4-{1-[2-(3,4-dichloro-phenyi)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyI-phenoxy)-2-methyl- propionic acid
2-(4-{1-[2-(4-ethyl-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl-phenoxy)-2-methyl- propionic acid 2-(4-{1-[2-(2-fluoro-4-trifluoromethyl-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl- phenoxy)-2-methyl-propionic acid
2-methyl-2-(2-methyl-4-{1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethoxy}-phenoxy)- propionic acid
2-methyl-2-(2-methyl-4-{1-methyl-1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]- ethoxy}-phenoxy)-propionic acid
2-methyl-2-(2-methyl-4-{1-methyl-1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethoxy}- phenoxy)-propionic acid
(R)-2-methyI-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] propoxy}- phenoxy)-propionic acid (S)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] propoxy}- phenoxy)-propionic acid
2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] butoxy}- phenoxy)-propionic acid
2-methyl-{2-methyl-2- (2-methyl-4- {1 -[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazoI-5-yl]- propoxy}-phenoxy)}-propionic acid
2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] pentyloxy}- phenoxy)-propionic acid
2-(4-{cyclopentyl-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-methoxy}-2-methyl- phenoxy)-2-methyl-propionic acid 2-methyl-2-(2-methyl-4-[4-methyl-2-(4-trifluoromethylphenyl)thiazol-5-yl- methoxy]phenoxy)propionic acid
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazoI-5-yl]-2-phenyl- ethoxy}-phenoxy)-propionic acid
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethylsulfanyl}- phenoxy)-propionic acid
2-methyI-2-(2-methyl-4-{1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethylsulfanyl}-phenoxy)- propionic acid
2-methyI-2-(2-methyl-4-{1-methyI-1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]- ethylsulfanyl}-phenoxy)-propionic acid 2-methyl-2-(2-methyl-4-{1-methyl-1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethylsulfanyl}- phenoxy)-propionic acid 2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]- propylsulfanyl}-phenoxy)-propionic acid
Most preferred compounds of formula 1 include: 2-{4-[({2-[2-fluoro-4-(trifluoromethyl)phenyl]-4-methyl-1 ,3-thiazol-5-yl}methyl)sulfanyl]-2- methylphenoxy}-2-methylpropanoic acid
(S)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyi)-thiazol-5-yl] ethoxy}- phenoxy)-propionic acid
(R)-2-methyl-2- (2-methyl-4- {1-[4-methyI-2- (4-trifluoromethylphenyl)-thiazol-5-yrj ethoxy}- phenoxy)-propionic acid
(R)-2-methyl-2- (2-methyI-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yi] propoxy}- phenoxy)-propionic acid
(S)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] propoxy}- phenoxy)-propionic acid
Those skilled in the art will recognize that stereocenters exist in compounds of formula (I). Accordingly, the present invention includes all possible stereoisomers and geometric isomers of formula (I) and includes not only racemic compounds but this invention is also intended to cover each of these isomers in their racemic, enriched, or purified forms. When a compound of formula (I) is desired as a single enantiomer, it may be obtained either by resolution of the final product or by stereospecific synthesis using an optically active catalyst or a catalytic system with optically active ligands or isomerically pure starting material or any convenient intermediate. Resolution of the final product, an intermediate or a starting material may be effected by any suitable method known in the art. See, for example, Stereochemistry of Carbon Compounds by E. L. Eliel (Mcgraw Hill, 1962) and Tables of Resolving Agents by S. H. Wilen. Additionally, in situations where tautomers of the compounds of formula (I) are possible, the present invention is intended to include all tautomeric forms of the compounds. In particular, in many of the preferred compounds of this invention the carbon atom to which R6 and R7 are bonded is chiral. In some of these chiral compounds the activities at the various PPAR receptors varies between the S and R isomers. Which of these isomers is preferred depends on the particular desired utility of the compound. In other words, even with the same compound, it is possible that the S isomer will be preferred for some uses, while the R isomer will be preferred for others.
The hPPAR agonists of formula (I) may be agonists of only one type ("selective agonists"), agonists for two PPAR subtypes ("dual agonists"), or agonists for all three subtypes ("pan agonists"). As used herein, by "agonist", or "activating compound", or "activator", or the like, is meant those compounds which have a pKi of at least 6.0 preferably at least 7.0 to the relevant PPAR, for example hPPARδ, in the binding assay described below, and which achieve at least 50% activation of the relevant PPAR relative to the appropriate indicated positive control in the transfection assay described below at concentrations of 10~5 M or less. More preferably, the agonists of this invention achieve 50% activation of at least one human PPAR in the relevant transfection assay at concentrations of 10"6 M or less. Preferably, the compounds of formula (I) are hPPAR agonists. More preferably the compounds are hPPARδ agonists. Most preferably they are dual agonists of hPPARδ and hPPARα, or pan agonists.
It will also be appreciated by those skilled in the art that the compounds of the present invention may also be utilised in the form of a pharmaceutically acceptable salt or solvate thereof. The physiologically acceptable salts of the compounds of formula (I) include conventional salts formed from pharmaceutically acceptable inorganic or organic acids or bases as well as quaternary ammonium acid addition salts. More specific examples of suitable acid salts include hydrochloric, hydrobromic, sulfuric, phosphoric, nitric, perchloric, fumaric, acetic, propionic, succinic, glycolic, formic, lactic, maleic, tartaric, citric, palmoic, malonic, hydroxymaleic, phenylacetic, glutamic, benzoic, salicylic, fumaric, toluenesulfonic, methanesulfonic, naphthalene- 2-sulfonic, benzenesulfonic hydroxynaphthoic, hydroiodic, malic, steroic, tannic and the like.
Other acids such as oxalic, while not in themselves pharmaceutically acceptable, may be useful in the preparation of salts useful as intermediates in obtaining the compounds of the invention and their pharmaceutically acceptable salts. More specific examples of suitable basic salts include sodium, lithium, potassium, magnesium, aluminium, calcium, zinc, N,N'-dibenzylethylenediamine, chloroprocaine, choline, diethanolamine, ethylenediamine, N-methylglucamine and procaine salts.
Those skilled in the art of organic chemistry will appreciate that many organic compounds can form complexes with solvents in which they are reacted or from which they are precipitated or crystallized. These complexes are known as "solvents". For example, a complex with water is known as a "hydrate". Solvates of the compound of formula (I) are within the scope of the invention. References hereinafter to a compound according to the invention include both compounds of formula (I) and their pharmaceutically acceptable salts and solvates.
The compounds of the invention and their pharmaceutically acceptable derivatives are conveniently administered in the form of pharmaceutical compositions. Such compositions may conveniently be presented for use in conventional manner in admixture with one or more physiologically acceptable carriers or excipients.
While it is possible that compounds of the present invention may be therapeutically administered as the raw chemical, it is preferable to present the active ingredient as a pharmaceutical formulation. The carrier(s) must be "acceptable" in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof. Accordingly, the present invention further provides for a pharmaceutical formulation comprising a compound of formula (I) or a pharmaceutically acceptable salt or solvate thereof together with one or more pharmaceutically acceptable carriers therefore and, optionally, other therapeutic and/or prophylactic ingredients.
The formulations include those suitable for oral, parenteral (including subcutaneous e.g. by injection or by depot tablet, intradermal, intrathecal, intramuscular e.g. by depot and intravenous), rectal and topical (including dermal, buccal and sublingual) administration although the most suitable route may depend upon for example the condition and disorder of the recipient. The formulations may conveniently be presented in unit dosage form and may be prepared by any of the methods well known in the art of pharmacy. All methods include the step of bringing into association the compounds ("active ingredient") with the carrier, which constitutes one or more accessory ingredients. In general the formulations are prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers or finely divided solid carriers or both and then, if necessary, shaping the product into the desired formulation.
Formulations suitable for oral administration may be presented as discrete units such as capsules, cachets or tablets (e.g. chewable tablets in particular for paediatric administration) each containing a predetermined amount of the active ingredient; as a powder or granules; as a solution or a suspension in an aqueous liquid or a non-aqueous liquid; or as an oil-in-water liquid emulsion or a water-in-oil liquid emulsion. The active ingredient may also be presented as a bolus, electuary or paste.
A tablet may be made by compression or moulding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine the active ingredient in a free-flowing form such as a powder or granules, optionally mixed with a other conventional excipients such as binding agents, (for example, syrup, acacia, gelatin, sorbitol, tragacanth, mucilage of starch or polyvinylpyrrolidone), fillers (for example, lactose, sugar, microcrystalline cellulose, maize-starch, calcium phosphate or sorbitol), lubricants (for example, magnesium stearate, stearic acid, talc, polyethylene glycol or silica), disintegrants (for example, potato starch or sodium starch glycollate) or wetting agents, such as sodium lauryl sulfate. Moulded tablets may be made by moulding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent. The tablets may optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active ingredient therein. The tablets may be coated according to methods well-known in the art.
Alternatively, the compounds of the present invention may be incorporated into oral liquid preparations such as aqueous or oily suspensions, solutions, emulsions, syrups or elixirs, for example. Moreover, formulations containing these compounds may be presented as a dry product for constitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents such as sorbitol syrup, methyl cellulose, glucose/sugar syrup, gelatin, hydroxyethylcellulose, carboxymethyl cellulose, aluminum stearate gel or hydrogenated edible fats; emulsifying agents such as lecithin, sorbitan mono- oleate or acacia; non-aqueous vehicles (which may include edible oils) such as almond oil, fractionated coconut oil, oily esters, propylene glycol or ethyl alcohol; and preservatives such as methyl or propyl p-hydroxybenzoates or sorbic acid. Such preparations may also be formulated as suppositories, e.g., containing conventional suppository bases such as cocoa butter or other glycerides.
Formulations for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non- aqueous sterile suspensions which may include suspending agents and thickening agents. The formulations may be presented in unit-dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in a freeze-dried (lyophilised) condition requiring only the addition of a sterile liquid carrier, for example, water-for-injection, immediately prior to use. Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets of the kind previously described. Formulations for rectal administration may be presented as a suppository with the usual carriers such as cocoa butter, hard fat or polyethylene glycol.
Formulations for topical administration in the mouth, for example buccally or sublingually, include lozenges comprising the active ingredient in a flavoured basis such as sucrose and acacia or tragacanth, and pastilles comprising the active ingredient in a basis such as gelatin and glycerin or sucrose and acacia.
The compounds may also be formulated as depot preparations. Such long acting formulations may be administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection. Thus, for example, the compounds may be formulated with suitable polymeric or hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt.
In addition to the ingredients particularly mentioned above, the formulations may include other agents conventional in the art having regard to the type of formulation in question, for example those suitable for oral administration may include flavouring agents. It will be appreciated by those skilled in the art that reference herein to treatment extends to prophylaxis as well as the treatment of established diseases or symptoms. Moreover, it will be appreciated that the amount of a compound of the invention required for use in treatment will vary with the nature of the condition being treated and the age and the condition of the patient and will be ultimately at the discretion of the attendant physician or veterinarian. In general, however, doses employed for adult human treatment will typically be in the range of 0.02-5000 mg per day, preferably 1-1500 mg per day. The desired dose may conveniently be presented in a single dose or as divided doses administered at appropriate intervals, for example as two, three, four or more sub-doses per day. The formulations according to the invention may contain between 0.1-99% of the active ingredient, conveniently from 30-95% for tablets and capsules and 3-50% for liquid preparations.
The compound of formula (I) for use in the instant invention may be used in combination with other therapeutic agents for example, statins and/or other lipid lowering drugs for example
MTP inhibitors and LDLR upregulators. The compounds of the invention may also be used in combination with antidiabetic agents, e.g. metformin, sulfonylureas and/or PPAR gamma, PPAR alpha or PPAR alpha/gamma agonists (for example thiazolidinediones such as e.g. Pioglitazone and Rosiglitazone). The compounds may also be used in combination with antihypertensive agents such as angistensin antagonists e.g. telmisartan, calcium channel antagonists e.g. lacidipine and ACE inhibitors e.g. enalapril. The invention thus provides in a further aspect the use of a combination comprising a compound of formula (I) with a further therapeutic agent in the treatment of a hPPAR mediated disease.
When the compounds of formula (I) are used in combination with other therapeutic agents, the compounds may be administered either sequentially or simultaneously by any convenient route.
The combinations referred to above may conveniently be presented for use in the form of a pharmaceutical formulation and thus pharmaceutical formulations comprising a combination as defined above optimally together with a pharmaceutically acceptable carrier or excipient comprise a further aspect of the invention. The individual components of such combinations may be administered either sequentially or simultaneously in separate or combined pharmaceutical formulations.
When combined in the same formulation it will be appreciated that the two compounds must be stable and compatible with each other and the other components of the formulation and may be formulated for administration. When formulated separately they may be provided in any convenient formulation, conveniently in such a manner as are known for such compounds in the art.
When a compound of formula (I) is used in combination with a second therapeutic agent active against the same hPPAR mediated disease, the dose of each compound may differ from that when the compound is used alone. Appropriate doses will be readily appreciated by those skilled in the art.
Compounds of this invention may be conveniently prepared by a general process wherein a moiety like A is coupled to an alcohol (B and D) using the Mitsunobu protocol (O. Mitsunobu, 1981 Synthesis, p 1 ) or by alkylation of A using a suitable non nucleophiiic base such as K2C03, Cs2C03 or NaH, with an alkyl halide (C, E and F). Note that this synthesis is preferably carried out with the acid group protected by R. Preferably, R is 1-6 alkyl which can be hydrolysed to give an acid of Formula (I), or if readily hydrolyzable, the resulting ester can be administered.
Figure imgf000014_0001
Figure imgf000014_0002
Some of the intermediates of type (A) are commercially available while others can be synthesised as outlined below. The synthesis of intermediates of type (B-F) are also illustrated below.
For example Y is S, Z is N, R1, R2, R3 and R6 are CH3, R7 is H, X2 is 0 and R8 is para-CF3:
Figure imgf000014_0003
Compounds of the invention may be made by an alternative method in which compounds of formula (G) are reacted with the phosphonium salts of formula (H) under standard Wittig conditions to afford the alkene of formula (J) which can be reduced with Pd/C under a hydrogen atmosphere to afford the ethyl ester of the compound of formula (I) which may be hydrolysed to produce the free acid.
Figure imgf000014_0004
Compounds of formula (H) may be prepared from the reaction between compounds of formula (C) and PPh3 in CH3CN at reflux for 1h. The following illustrates Intermediates and Examples of Formula 1 which should not be construed as constituting a limitation thereto.
The structures of the compounds were confirmed either by nuclear magnetic resonance (NMR) or mass spectrometry (MS). 1 H NMR spectra were recorded on a Brucker 300MHz spectrometer at ambient temperature. NMR shifts (δ) are given in parts per million (ppm), "mp" is melting point and is given in °C. Column chromatography was carried out using the technique described by W.C. Still et al, J.Org.Chem. 1978, 43, 2923-2925 on Merck silica gel 60 (40-63 μM).
Compounds used as starting materials are either commercially available compounds or known compounds.
Abbreviatioi is : tic : thin layer chromatography e.e. : enantiomeric excess
DMSO-d6 : deutorated dimethylsulfoxide
CDCI3 : deutorated chloroform
CD3OD : deutorated methanol
C-6H12 : cyclohexane
DCC : dicycohexylcarbodiimide
DMAP : 4-dimethyIaminopyridine
DMF : N,N-dimethylformamide
Et20 : diethylether
EtOAc : Ethylacetate
MeOH : Methanol
PBu3 : Tributylphosphine
PCC : Pyridinium chlorochromate
Rf : retention fraction
Rt : retention time
TMAD : Azodicarboxylic acid bis[dimethylamide]
THF : tetrahydrofuran min: minutes br : broad s: singlet d: doublet dd : doublet of doublet t : triplet q: quartet m : multiplet Intermediate 1 :
Figure imgf000016_0001
To a well stirred solution of LiAIH4 (1.52 g, 40 mmol) in dry THF (50 mL) at 0°C, was slowly added a solution of ethyl 4-methyl-2-[4-(trifluoromethyl)-phenyl]-thiazole-5-carboxylate (12.6 g, 40 mmol) in dry THF (50 mL). The mixture was stirred at room temperature for 2h. The reaction was quenched by slow addition at 0 °C of water (2 mL), 5N NaOH (2 mL) and water (6 mL). The precipitate was filtered, washed with EtOAc, MeOH, CH2CI2and THF. After evaporation, a yellow solid was obtained, that was crystallised from MeOH-water to afford intermediate 1 depicted above (9.90 g, 36 mmol, 90%) as a yellow solid mp 120-122 °C.
Intermediate 2:
Figure imgf000016_0002
To a solution of 2-fluoro-4-(trifluoromethyl)benzonitrile (5.2g, 27.5mmol) in 50mL methanol was added 10ml of water (137.5mmol) followed by NaSH«H20 (7.7g, 137.5mmol). After heating at 50°C for 12 hours, the solvent was removed in vacuo and the residue treated with water (200mL) and extracted with EtOAc (2 x 150mL). The organic layers were dried (MgS04) and the solvent evaporated to give crude residue which was purified on a Biotage FlashElute with a 40M silica cartridge, eluting with hexane / ethyl acetate (4:1) to yield the title compound as a yellow solid (3.27g, 53%).
MS C8H6F4NS: m/z 224 (M+1 ); HPLC RT 2.013 (C18 4.6x60mm, 1 % MeOH / 0-90% CH3CN / H20 (0.1% TFA) / (50mM TEA / TFA), 4min @ 3mL / min @ 254 / 220nm).
Intermediate 3:
Figure imgf000016_0003
Intermediate 2 was treated with ethyl 2-chloro-3-oxobutanoate in refluxing ethanol overnight and evaporated. The residue was passed through a plug of silica gel with hexane:ethyl acetate (4:1 ) to afford the title compound as a light yellow solid after evaporation (71 %).
MS C14H12F4N02S: m/z 333 (M+1 )
Intermediate 4 :
Figure imgf000016_0004
To a solution of NaOEt (9.94g, 14.6mmol) in EtOH (50mL), under a nitrogen atmosphere, were added Ethyl formate (11.81 mL,14.6mmol) and chloro acetic acid ethyl ester (10.96mL,14.6mmoI) in solution in 50mL of dry Et20. The mixture was stirred at rt for 20hours and filtered after addition of 50mL of dry Et20. The resulting solid was dissolved in 100mL EtOH and after addition of 4-trifluoro methyl thiobenzamide (3g, 14.6mmol) stirring to reflux was accomplished for 20 hours. EtOH was removed under reduced pressure and 250mL of CH2CI2 and 50 mL of water were added. The organic layer was separated and dried over Na2S04, filtered, concentrated to dryness and purified by flash chromatography C6H 2/Et0Ac (80:20) to afford the title compound (750mg, 2.49mmol) as a white powder in a 17% yield. GC/MS C13H10F3NO2S: m/z 301
Figure imgf000017_0001
Intermediate 3 was reacted as described in a general LiAIH4 reduction procedure to afford the title compound as a light yellow solid (83%) MS C12H9F4NOS: m/z 291 (M+1)
Intermediate 6:
Figure imgf000017_0002
Intermediate 5 was reacted with mesylchloride to afford the title compound as a light yellow solid (100%).
Rf of starting alcohol in 3:1 hexanes / ethyl acetate 0.25
Rf of chloride in 3:1 hexanes / ethyl acetate 0.75
Intermediate 7:
Figure imgf000017_0003
Intermediate 6 was treated with 2-methyl-4-suIfanylphenol as described in a general alkylation procedure to afford the title compound as a yellow oil (0.242g, 49%).
1H NMR (CDCls): δ 2.22 (s, 3H), 2.25 (s, 3H), 4.15 (s, 2H), 6.69 (d, 1 H), 7.13 (d, 1 H), 7.20 (s, 1 H), 7.50 (m, 2H), 8.39 (t, 1 H), MS C19H16F4NOS2: m/z 414 (M+1).
Intermediate 8:
Figure imgf000017_0004
To a solution of Intermediate 1 (75.5g, 0.276 mmol, 1 eq) in CH2CI2 was added pyridinium chlorochromate (119g, 0.552 mmol, 2 eq). Then the resulting mixture was stirred at room temperature for 3 hours. The mixture was decanted one night and then filtered over celite and evaporated off. The residue was purified by flash chromatography using CH2Cl2 as eluent to give the title compound as a yellow solid (46 g, 0.17 mmol) in a 61.5% yield.
GC/MS: C12H8F3NOS: m/z 271
Intermediate 9 :
Figure imgf000018_0001
To a solution of intermediate 4 (0.8g, 2.65mmol) was added dropwise LiAIH4 (2.7mL / 1 N in THF, 2.7mmol). The resulting mixture was stirred 30 min at rt and the reaction was quenched by the cautious addition of NH CI and water. The precipitate was filtered, the filtrate was concentrated under vacuo and the residue obtained was flash chromatographed CH2CI2/MeOH (99:1 ) to afford the title compound (500mg, 1.93mmol) as a white solid in a 73% yield.
1H NMR (CDCIs) δ: 3.48 (br t, 1 H), 4.73 (br d, 2H), 7.52 (m, 3H), 7.82 (d, 2H)
Intermediate 10 :
Figure imgf000018_0002
To a solution of intermediate 9 (500mg, 1.93mmol) in CH2CI2 (20mL) was added PCC (830mg, 2eq.). After stirring for 2 hours at rt the solvent was evaporated off and the residue flash chromatographed with CH2CI2 to give the title compound (460mg, 1.78mmol) as a white powder in a 93% yield.
1H NMR (CDCI3) δ: 7.68 (d, 2H), 8.07 (d, 2H), 8.41 (s,1 H), 10.01(s,1 H)
Intermediate 11 :
Figure imgf000018_0003
A solution of 4-trifluoromethyl-thiobenzamide (5g, 24.3mmol) and 3-chloro-pentane-2,4- dione (3.2mL, 24.3mmoles) in 50 mL of EtOH was heated to reflux during 18 hours and evaporated to dryness. The residue was diluted with 250 mL of CH2CI2 and washed with 50mL of saturated solution of NaHC03. The organic phase was separated and dried over Na2S04. After filtration and concentration under vacuo the residue was purified by flash chromatography (CH2Cl2) to give the title compound as a tan powder (6.3g, 22mmol) in a 90% yield. GC/MS C13H10F3NOS : m/z 285 Intermediate 12:
Figure imgf000019_0001
A solution of 3-fIuoro-4-trifluoromethyl-thiobenzamide (540mg, 2.42mmol) and 3-chloro- pentane-2,4-dione (577μL, 4.9mmoles) in 50 mL of EtOH was heated to reflux during 48 hours and evaporated to dryness. The residue was diluted with 250 mL of CH2Cl2 and washed with
50mL of a saturated solution of NaHC03. The organic phase was separated and dried over Na2S04. After filtration and concentration under vacuo the residue was purified by flash chromatography (CH2CI2) to give the title compound as a tan powder (500mg, 1.65mmol) in a 68% yield. GC/MS C13H9F4NOS : m/z 303
Figure imgf000019_0002
Intermediate 13 :
A solution of 4-ethyl-thiobenzamide (2g, 12mmol) and 3-chloro-pentane-2,4-dione (2.2mL, 14 mmol) in 30 mL of EtOH was heated to reflux during 18 hours and evaporated to dryness. The residue was diluted with 250 mL of CH2CI2 and washed with 50mL of a saturated solution of
NaHC03. The organic phase was separated, dried over Na2S04, filtered and concentrated under vacuo to give the title compound (2.94g, 12mmol) in a quantitative crude yield.
1H NMR (CDCI3) δ: 1.15 (t,3H), 2.52 (s, 3H), 2.61 (q,2H), 2.72(s,3H), 7.20 (d, 2H), 7.85(d,2H)
Intermediate 14 :
Figure imgf000019_0003
A solution of 3,4-dichIoro-tfιiobenzamide (1.5g, 7.3mmol) and 3-chloro-pentane-2,4-dione (1.2mL, 11 mmol) in 30 mL of EtOH was heated to reflux during 18 hours and evaporated to dryness. The residue was diluted with 250 mL of CH2CI2 and washed with 50mL of saturated solution of NaHC03. The organic phase was separated, dried over Na2S04, filtered and concentrated under vacuo to give the title compound as an off white powder (1.5g, 5.2mmoI) in a 71 % yield after trituration of the residue in water and washing with pentane.
1H NMR (CDCI3) δ: 2.52 (s, 3H), 2.72(s, 3H), 7.47 (d, 1 H), 7.74 (dd,1 H), 8.04 (d, 1 H)
Intermediate 15 :
Figure imgf000020_0001
A solution of 4-chIoro-thiobenzamide (5g, 29mmol) and 3-chloro-pentane-2,4-dione (3.2mL, 27mmol) in 50 mL of EtOH was heated to reflux during 5 hours and evaporated to dryness. The residue was diluted with 250 mL of CH2CI2 and washed with 50mL of saturated solution of NaHC03. The organic phase was separated and dried over Na2S04. After filtration and concentration under vacuo the residue was purified by flash chromatography (CH2CI2) to give the title compound as (7.2g, 28.5mmol) in a quantitative yield.
1H NMR (CDCIs) δ: 2.54 (s, 3H), 2.83 (s, 3H), 7.41 (d, 2H), 8.01 (d, 2H)
Intermediate 16:
Figure imgf000020_0002
To a solution of intermediate 8 (1.9g, 7mmol) in 25 mL of THF was added slowly at -10°C, a solution of 1.4M methylmagnesium bromide in tetrahydrofuran (7mL, 11.9 mmol, 1.4 eq). The mixture was naturally warmed at room temperature and then stirred for 1 ,5 hour. The resulting mixture was quenched with saturated NH4CI solution (100mL) and extracted with Ethyl Acetate (2 x 250mL). The organic phase was washed with brine and water, and then dried over Na2S04 and evaporated off to give the title compound as a yellow solid (1.9g, 6.96 mmol) in a 99% crude yield.
GC/MS C13H12F3NOS: m/z 287 mp: 147°C
Intermediate 17 :
Figure imgf000020_0003
To a solution of the intermediate 10 (1.35g, 5.25mmol) was added slowly at -10°C, a solution of 1.4M methylmagnesium bromide in tetrahydrofuran (4.1 ml, 5.8 mmol, 1.1 eq). The mixture was naturally warmed at room temperature and then stirred for 1 ,5 hour. The resulting mixture was quenched with saturated NH Cl solution (100ml) and extracted with Ethyl Acetate(2 x 250ml). The organic phase was washed with brine and water, and then dried over Na2S04 and evaporated off to give the title compound as an off white solid (700mg, 2.58 mmol) in a 49 % yield after purification by flash chromatography CH2CI2/MeOH (99:1 ). GC/MS: C12H10F3NOS m/z= 273
Intermediate 18 :
Figure imgf000021_0001
To a solution of intermediate 11 (5g, 17.5mmol) and (S)-2-methyl-CBS-oxazaborolidine (3.5mL, 3.5mmol , 1N solution in toluene) in anhydrous THF was added slowly at 0°C, a solution of borane-methylsulfide complex(10.5 mL, 21 mmol, 2M solution in THF). The solution was stirred 2,5 hours and a tic monitoring of an hydrolyzed aliquot indicated that the reaction was completed. 20 mL of MeOH and 100 mL of 0.5 N HCI were added to the solution to quench the reaction. EtOAC extraction ( 3 x 200mL) and acidic washing with diluted HCI (3 x 50 L) afforded after drying over Na2S0 , filtration and concentration under vacuo , 5g of a white solid.
Recrystallization in Hexane /EtOH (200mL / 6mL) gave after filtration 1.12g of a racemic powder. The filtrate was evaporated to dryness and the resulting yellow solid was triturated with 100 mL of hexane to yield 3.5g of a white solid after filtration with an ee = 95.5 %.
1H NMR(CDCI3, 300Mhz) δ: 1.4(d, 3H), 2.26 (s, 3H), 5.03 (q,1 H), 7.06 (s,1 H), 7.46 (d, 2H), 7.8 (d, 2H)
HPLC Chiralpak AD-RH (4.6x150mm, 65% CH3CN / 35% H20, 0.3mL/min)
Rt : 17.0 min
[α]25 D = + 38.1 (c= 0.25 / CHCI3) for ee = 95.5 %
Intermediate d
Figure imgf000021_0002
The same experimental procedure as for intermediate 18 was applied to a 3g quantity of the methylketone using the ( R)-2-MethyI-CBS-oxazaborolidine. The title compound (1.7g; 5.9 mmol) was obtained as a white powder in a 56% yield and with an enantiomeric excess of 79.4% as determined by HPLC. To increase the enantiomeric excess, the resulting alcohol (1.7g, 5.9 mmol) was coupled with the (R)-(-)-α Methoxyphenylacetic acid ( 1.1 g; 6.5 mmol) with DCC (1.5g) and a catalytic amount of DMAP. The two diatereomeric esters had ΔRf = 0.1 which allowed the isolation of the less polar fraction, the major one (2.35g, 5.4 mmol) after flash chromatography using Petroleum Ether/ EtOAc (85:15). Finally this ester in solution in 50 mL of EtOH was saponified at 0°C using 1N NaOH (5.7mL, 5.7mmoI). After completion the reaction was quenched by dropwise addition at 0°C of 1 N HCI (5.7mL). After removing EtOH under reduced pressure, extraction with Et20 (200mL) washing of the organic phase with 25mL of saturated NaHC03 afforded the title compound (1.4g, 4.8 mmol) in 86% yield as a pale oil with an e.e.=98%. H NMR(CDCI3, 300Mhz) δ: 1.60 (d, 3H), 2.44 (s, 3H), 5.21 (q, 1 H), 7.66 (d, 2H), 8.0 (d, 2H)
HPLC Chiralpak AD-RH (4.6x150mm, 65% CH3CN / 35% H20, 0.3mL/min)
Rt : 15.54 min
[α]25 D = - 31 (c = 0.32 / CHCI3) for ee = 86%
Intermediate 20 :
Figure imgf000022_0001
To a solution of intermediate 12 (500mg, 1.65mmol) in MeOH (25mL) was added at once NaBH4 (69mg,1.7mmol). The solution was stirred 30 min and concentrated to dryness. Hydrolysis with 1 N HCI (5mL), followed by Et20 extraction (2x75mL),drying of the organic phase over Na2S04, filtration and concentration under vacuo gave a residue which was flash chromatographed CH2CI2/ MeOH (99:1 ) to afford 380 mg of the title compound.
1H NMR(CDCl3) δ:1.53 (d, 3H), 2.39 (s, 3H), 5.12 (q, 1 H), 7.15 (s, 1 H), 7.57 (m, 1 H), 7.68 (m, 2H)
Figure imgf000022_0002
Intermediate 21 :
To a solution of methylketone intermediate 13 (3.5g, 14.2mmol) in EtOH (50mL) was added at once NaBH ( 1.1g, 25mmol, 2eq.) at 0°C. After stirring 3 hours at room temperature EtOH was removed under reduced pressure and 1 N HCI was added. The resulting precipitate was filtered off, washed with water and dried under vacuum to afford the title compound (1.9g, 7.6mmol) in a 54% yield as a white solid.
1H NMR (CDCi3) δ: 1.22 (t, 3H), 1.54(d, 3H), 2.37 (s, 3H), 2.64(q, 2H), 5.14(q,1 H), 7.20 (d, 2H), 7.76(d, 2H)
Figure imgf000022_0003
Intermediate 22 :
To a solution of methylketone intermediate 14 (1.7g, 5.9mmol) in EtOH (25mL) was added at once NaBH4 ( 450mg, 12mmol, 2eq.) at 0°C. After stirring 3 hours at room temperature EtOH was removed under reduced pressure and 1 N HCI was added. The resulting precipitate was filtered off, washed with water and dried under vacuum to afford the title compound (1.58g, 5.48mmol) in a 92% yield as a white solid.
1H NMR (CDCI3) δ: 1.50(d, 3H), 2.01 (d,1 H), 2.35 (s, 3H), 5.12(m,1 H), 7.37 (d, 1 H, J = 8.48 Hz), 7.60(dd,1 H, J = 8.3 Hz and 2.07 Hz), 7.92(d,1 H, J = 2.1 Hz)
Intermediate 23 :
Figure imgf000023_0001
To a solution of methylketone intermediate 15 (2.5g, 9.9mmol) in EtOH (25mL) was added at once NaBH4 ( 750mg, 20mmol, 2eq.) at 0°C. After stirring 3 hours at room temperature EtOH was removed under reduced pressure and 1 N HCI was added. The resulting precipitate was filtered off, washed with water and dried under vacuum to afford the title compound (2.4g, 9.4mmol) in a 95% yield as a white solid.
1H NMR (CDCI3) δ: 1.56(d,3H, J= 6Hz), 2.39 (s, 3H), 5.16(m,1 H), 7.37 (d, 2H, J= 8.0Hz), 7.79(d,2H, J= 8.0Hz )
Intermediate 24 :
Figure imgf000023_0002
To a solution of ethyl-4-methyl-2-[4-(trifluoromethyl)-phenyl]-thiazole-5-carboxylate (2g, 6.4mmol) was added slowly at -10°C, a solution of 1.4M methylmagnesium bromide in toluene (10 mL, 14 mmol, 2 eq). The mixture was naturally warmed at room temperature and then stirred for 72 hours. The resulting mixture was quenched with saturated NH4CI solution (100mL) and extracted, with EtOAc (2 x 250mL). The organic phase was washed with brine and water, and then dried over Na2S0 and evaporated off to give the title compound as a yellow solid (1g, 3.3 mmol) in a 52% yield.
1H NMR (CDCI3) δ: 1.70 (s, 6H), 2.65 (s, 3H), 7.60 (d, 2H), 7.95 (d, 2H)
Intermediate 25 :
Figure imgf000023_0003
To a solution of intermediate 4 (750mg, 2.5mmoI) was added slowly at -10°C, a solution of 1.4M methylmagnesium bromide in toluene (8.95mL, 12.5 mmol, 5 eq). The mixture was naturally warmed at room temperature and then stirred for 24 hours. The resulting mixture was quenched with saturated NH4CI solution (100mL) and extracted with EtOAc (2 x 250mL). The organic phase was washed with brine and water, and then dried over Na2S04 and evaporated off to give the title compound (460mg, 1.6 mmol) in a 64% yield after flash chromatography CH2CI2/MeOH (99:1 ). GC/MS : C13H12F3NOS m/z : 287
Intermediate 26:
Figure imgf000024_0001
To a solution of intermediate 8 (4.05g, 15mmol) in 50 mL of THF was added slowly at - 10°C, a solution of 3M ethylmagnesium bromide in Et20 (5.5 mL, 16.5mmol, 1.1 eq). The mixture was naturally warmed at room temperature and then stirred for 1 ,5 hour. The resulting mixture was quenched with saturated NH4CI solution (100mL) and extracted with ethyl Acetate. The organic phase was washed with brine and water, and then dried over Na2S04 and evaporated off. The residue was taken up with a mixture of isopropyl ether and petroleum ether. The white solid obtained was filtered to give the title compound (4.31 g, 14.3 mmol) in a 95% yield. GC/MS: C14H14F3NOS: m/z 301 mp: 104-106°C
Intermediate 27:
Figure imgf000024_0002
Intermediate 26 ( 8.23g,27.3 mmol) was added to a mixture of (R)-(-)-α- Methoxyphenylacetic acid ( 5g, 30 mmol), DCC (6.18g, 30 mmol) and DMAP (catalytic) in 250 ml THF The mixture was stirred at room temperature 24 hours and passed through a plug of silica gel. The filtrate was concentrated to dryness and flash chromatographed using Petroleum ether / EtOAc (95:5). The less polar fraction was collected to afford 2.9g of the title compound (2.9g, 6mmol).
1H NMR(CDCI3) δ: 0.73 (t, 3H), 1.83 (m,2 H), 2.49 (s, 3H), 3.37 (s, 3H), 4.74 (s, 1 H), 5.97 (t, 1 H), 7.33 (m, 3H), 7.43 (m, 2H), 7.66 (d, 2H), 8.00 (d, 2H)
Intermediate 28 :
Figure imgf000024_0003
To a well-stirred solution of Intermediate 27 (2.75g, δmmol) in THF/EtOH was added dropwise at 0°C a solution of 18 mL of 1 N NaOH in 15 mL of water. After 5 min the cleavage was completed and slowly at 0°C was added 18mL of 1 N HCI in 15 ml H20. The organic solvents were removed under reduced pressure and extraction with EtOAc ( 250 mL) washing with brine (25 mL) and drying over Na2S04 gave a residue which was chromatograhed CH2CI2/EtOAc (90:10) to afford in a 82% yield (1.49g, 3.3mmol) the title compound with an e.e. = 98%.
GC/MS : C14H14F3NOS m/z 301
[α]25 D= -10 (c = 0.279, CHCI3) for e.e. = 98%
HPLC Chiralpak AD-RH (4.6x150mm, 65% CH3CN / 35% H20, 0.3mL/min)
Rt : 21.2 min
Figure imgf000025_0001
The same esterification protocol as described for intermediate 27 was applied with 10 g of racemic Intermediate 26 and 6g of (S)-(+)-α-Methoxyphenylacetic acid to afford 2.85g of the less polar diastereomer.
1H NMR(CDCI3) δ: 0.73 (t, 3H), 1.82 (m, 2H), 2.49 (s, 3H), 3.37 (s, 3H), 4.74 (s,1 H), 5.97 (t, 1 H), 7.33 (m, 3H), 7.43 (m, 2H), 7.66 (d, 2H), 8.0(d, 2H)
Intermediate 30: H0 H 0F'
The same saponification protocol as used to obtain Intermediate 28 was applied to intermediate 29 (2.85g, 6.35 mmol) to afford the title compound (1.58g,5.25mmol) as a pale yellow oil in a 82.5% yield and with an e.e. = 98%
1H NMR(CDCI3) δ: 0.98 (t, 3H), 1.96 (m, 2H), 2.45 (s, 3H), 4.92 (t, 1 H), 7.66 (d, 2H), 8.0 (d, 2H)
[α]25 D = + 11 (c = 0.29, CHCI3) for e.e. = 98%
HPLC Chiralpak AD-RH (4.6x150mm, 65% CH3CN / 35% H20, 0.3mL/min) Rt : 25.04 min
Intermediate 31 :
Figure imgf000025_0002
To a solution of intermediate 8 (2.71 g, 10mmol) was added slowly at -12°C, a 2M solution of isopropylbromide magnesium in THF (5.5 ml, 11 mmol). The mixture was naturally warmed at room temperature and then stirred for 1 ,5 hour. The resulting mixture was quenched with saturated NH CI solution (100ml) and extracted with EtOAC (2 x 150ml). The organic phase was washed with brine and water, and then dried over Na2S04 and evaporated off to give the title compound (2g, 6.34 mmol) in a 63.5% yield as a slightly yellow powder after crystallisation in hexane /Ethanol.
1H NMR(CDCI3, 300MHz) δ: 0.8 (d, 3H),1.05(d, 3H),1.9(m, 1 H), 2.35(s, 3H), 4.55 (d, 1 H), 7.6(d, 2H), 7.95(d, 2H)
Intermediate 32:
Figure imgf000026_0001
To a solution of intermediate 8 (5.42g, 20mmol) in 50 mL of THF was added slowly at -
78°C, a 1 M solution of allylmagnesium bromide in Et20 (22mL, 22mmol, 1.1 eq). The mixture was stirred 30 minutes at -78°C and was naturally warmed at RT and then stirred for 1 hour. The resulting mixture was quenched with saturated NH4CI solution (100mL) and extracted with ethyl acetate. The organic phase was washed with brine and water, and then dried over Na2S04 and evaporated off. The residue was taken up in heptane to yield the title compound after filtration as a white solid (5g, 16 mmol) in a 80% yield.
LC/MS: Cl5H15F3NOS: m/z 314.00 (M+1 )
Intermediate 33 :
Figure imgf000026_0002
To a solution of intermediate 8 (813mg, 3mmol) was added slowly at -78°C, a 2M solution of butyl lithium in tetrahydrofuran (1.6 mL, 3 mmol). The mixture was naturally warmed at room temperature and then stirred for 1 ,5 hour. The resulting mixture was quenched with saturated NH4CI solution (100mL) and extracted with EtOAc (2x100mL). The organic phase was washed with brine and water, and then dried over Na2S0 and evaporated off to give the title compound as a yellow solid (770mg, 6.96 mmol) in a 99 % crude yield.
1H NMR(CDCI3, 300MHz) δ: 0.8 (t, 3H),1.25 (m, 4H),1.7 (m, 1 H),1.8 (m, 1 H), 2.3 (s, 3H), 2.8 (br s, 1 H), 4.9 (t, 1 H),7.55 (d, 2H), 7.9 (d, 2H) mp 72-74°C Intermediate 34 :
Figure imgf000027_0001
To a solution of intermediate 8 (5g, 18.45mmol) was added slowly at 0°C, a 2M solution of cyclopentylmagnesium bromide in Et20 (11 mL, 22 mmol). The mixture was naturally warmed at room temperature and then stirred for 1 ,5 hour. The resulting mixture was quenched with saturated NH4CI solution (100mL) and extracted with EtOAc(2 x 250mL). The organic phase was washed with brine and water, and then dried over Na2S0 , evaporated off to give the title compound (2.2g, 6.4 mmol) in a 35% yield after flash chromatography using C6H12/EtOAc (85:15)
LC/MS C17H19F3NOS : m/z 342 (M+1 )
Intermediate 35:
Figure imgf000027_0002
To a solution of intermediate 8 (1.4g, 5.17mmol) in 50 mL of THF was added slowly at - 78t'C, a 1 M solution of phenylmagnesium bromide in THF (5.7mL, 5.7mmol. 1.1 eq). The mixture was stirred 30 min at ~78°C and was naturally warmed at room temperature and then stirred for 2 hours. The resulting mixture was quenched with saturated NH4CI solution and extracted with ethyl acetate. The organic phase was washed with water, dried over Na2S04, evaporated off and the residue was crystallized in hexane. The white solid obtained was filtered and washed with hexane to give the title compound (1.7g, 4.9 mmol) in a 86% yield.
GC/MS: C18H14F3NOS m/z 349
Intermediate 36:
Figure imgf000027_0003
To a solution of intermediate 8 (4.48g, 16.5mmol) was added slowly at -10°C, a solution of 1.3M benzylmagnesium chloride in tetrahydrofuran (20 ml, 26.5 mmol, 1.6 eq). The mixture was naturally warmed at room temperature and then stirred for 1 ,5 hour. The resulting mixture was quenched with saturated NH4CI solution (100mL) and extracted with Ethyl Acetate(2 x 250mL). The organic phase was washed with brine and water, and then dried over Na2S04 and evaporated off to give a pale yellow solid. After sonication in petroleum ether and filtration, the title compound (2.8g, 7.7 mmol) was obtained in a 48 % yield as a white powder. LC/MS: C19H17F3N0S m/z 364.13 (M+1) mp: 126-129°C
Intermediate 37:
Figure imgf000028_0001
A suspension of 20g (0.133 mole) of 4-acetyl-2-methylphenol and 30g of potassium carbonate ( 0.2 mole) in 500 mL of acetone was heated at reflux for 24 hours. 30mL of ethyl 2- bromo-2-methylpropionate in solution in 30 mL of acetone was added dropwise. The mixture was stirred at reflux during 20 hours and a tic monitoring showed that the reaction was not complete. Then two supplementary equivalent portions of potassium carbonate and halide were added with a 7 hours interval. The mixture was stirred for 24 hours at reflux and stirred at room temperature for 2 days then filtered off and concentrated under vacuo. The residue was taken up with 300 mL of EtOAc and washed with 10OmL of 1 N NaOH and 10OmL of brine. The organic layer was dried over Na2S0 , filtered and concentrated under vacuo to give 32.6g of the title compound as a yellow oil in a 92% yield. H NMR (CDCIs): δ 1.19 (t, 3H), 1.63 (s, 6H), 2.24(s, 3H), 2.50 (s, 3H), 4.20 (q, 2H), 6.58 (d, 1 H), 7.67 (d, 1 H), 7.75 (s,1 H),
Intermediate 38:
Figure imgf000028_0002
To a solution of 30g (0.11 mole) of intermediate 37 in 300 mL of CH2CI2 was added at once 2.2g (0.011 mole) of p-toluenesulfonic acid and 35.9g (0.125 mol) of m-chloroperbenzoic acid. The solution was heated at 50°C for 21 hours then filtered and washed consecutively with a solution of 20 of Kl in 200mL of water, 20g of Na2S03 in 200 mL of water, 150 mL of 1 N NaOH and 10OmL of brine. The organic layer was dried over Na2S04, filtered and concentrated under vacuo to afford 28.54g of the expected compound in a 89% yield.
GC/MS C15H20O5: m/z 280
Figure imgf000028_0003
To a solution of 25g (0.089 mol) of intermediate 38 in 250mL of anhydrous EtOH was added 9.1g of NaOEt (0.134 mol). The solution was heated at 50°C for 6 hours and concentrated under vacuo. The residue was taken up with 200mL of water and acidified cautiously to pH = 1 with 150mL of 1 N HCI. Extraction with 3 x 200mL of EtOAc and drying of the gathered organic layers over MgS04 led after filtration and evaporation under vacuo to 21.62g of the title compound. GC/MS C13H1804: m/z 238
Intermediate 40:
To a cold (0°C) stirred solution of intermediate 1 (8.2g, 30 mmol) and Et3N (6.07g, 8.36mL, 60 mmol), in dry CH2CI2 (120mL) was slowly added MeS02CI (5.49g, 3.71 mL, 48 mmol). After 2 hours at 0°C more Et3N (6 mmol) and MeS02CI (4.8 mmol) were added. After 2 more hours a tic (hexane : EtOAc, 1 :1 ) showed complete reaction. The reaction mixture was diluted with CH2CI2 (120mL) and washed with NaHC03 (sat.) (2 x 240mL) and water (2 x 240mL), dried, filtered and evaporated to afford the title compound (8.0g, 27 mmol, 90%) as a yellow solid.
Figure imgf000029_0002
Intermediate 41
A solution of 2-fluoro-4-trifluoromethyl-thiobenzamide (1.02g, 4.57mmol) and 3-chloro- pentane-2,4-dione (654μL, 5.5mmol) in 30 mL of EtOH was heated to reflux during 18 hours. A supplementary 1.2equivalent of 3-chloro-pentane-2,4-dione (654μL, 5.5mmol) was added, the reaction heated to reflux for an additional 18 hours and evaporated to dryness. The residue was diluted with 250 mL of CH2CI2 and washed with 50mL of saturated solution of NaHC03. The organic phase was separated, dried over Na2S04, filtered and concentrated under vacuo to give the title compound (850mg, 12mmol) in a 61%yield as a yellow solid after flash chromatography CH2CI2.
GC/MS C13H9F4N0S m/z 303
Intermediate 42
Figure imgf000029_0003
To a solution of methylketone intermediate 41 (850mg, 2.8mmol) in EtOH (50mL) was added at once NaBH ( 117mg, 3mmoi, 1.1eq.) at 0°C. After stirring 30 minutes at room temperature MeOH was removed under reduced pressure, 1N HCI was added and extraction with Et20 (2 x 100mL) followed by drying over Na2S04 and filtration afforded the title compound (770mg, 2.53mmol) in a 90% yield as a pale yellow solid.
GC/MS C13H11F4NOS m/z 305 Intermediate 43:
Figure imgf000030_0001
To a solution of chlorosulfonic acid ( 90 mL, 4.4eq.) was added dropwise at 0°C in 1 hour 2- Methyl-2-o-tolyloxy-propionic acid ethyl ester (68g, 0.306 mol). After stirring for 1 hour at 0°C and an additional 30 min at 15°C the mixture was poured into crushed ice and the precipitate thus obtained was filtered and washed with cold water. The title compound was obtained as an off white powder (62.2g, 0.194 mol) in a 63% yield.
GC/MS : C13H17CI05S m/z :320.5
Intermediate 44 :
Figure imgf000030_0002
In a three neck round bottom flask under a nitrogen atmosphere containing Zn (13.1 g,
0.2moi, 3.5eq) in dry EtOAc (150mL) was added intermediate 43 (18.32g, 0.057mol). After stirring for 30 min, dichloromethylsilane (24.4 mL, 0.2 mol, 3.5 eq) was added slowly in 90 min at 70°C. After stirring for 5 hours at 70°C a supplementary portion of Zn (3g, 45mmol) was added to complete the reaction. Then the resulting mixture was stirred overnight .filtered and the zinc precipitate washed with EtOAc. A back extraction was carried out on the filtrate with 1N NaOH
(3x300mL) and the gathered basic phases were washed with EtOAc (400mL). Acidification of basic aqueous phases to pH = 3-4 with concentrated HCI, extraction with EtOAc (3 x 300mL), drying of the organic phase over MgS0 , filtration and concentration to dryness gave a colorless oil. The title compound (10.01g, 39.4mmol) was obtained after flash chromatography C6Hι2/EtOAc (90:10) as a colorless oil in a 69% yield.
GC/MS : Cι3H1803S m/z :254
Example 1 :
Figure imgf000030_0003
2-{4-[({2-[2-fluoro-4-(trifluoromethyl)phenyl]-4-methyl-1 ,3-thiazoi-5-yl}methyl)sulfanyl]-2- rnethylphenoxy}-2-methylpropanoic acid
To a 25ml round-bottom flask equipped with a magnetic stir-bar and N2 inlet was added intermediate 7 (240mg, 0.58 mmol, 1eq) in acetone (4ml) followed by the addition of 2- trichloromethyl-2-propanol (210 mg, 1.18 mmol, 2eq). The reaction was cooled to 0°C and then NaOH (pellets, 190 mg, 4.8 mmol, 8eq) were added. The reaction mixture warmed to room temperature and stirred at room temperature overnight after which the acetone was removed in vacuo and the resulting residue was partitioned between EtOAc and water acidified to pH2 with cone. HCI. The phases were then separated and the organic fraction was washed with brine, dried over MgS04 and concentrated in vacuo to yield after chromatography (0.05g, 17%) of the title compound as a cream colored solid.
1H NMR (CD3OD): δ 1.59 (s, 6H), 2.17 (s, 3H), 2.20 (s, 3H), 4.24 (s, 2H), ), 6.72 (d, 1H), 7.12 (d, 1 H), 7.20 (s, 1 H), 7.65 (m, 2H), 8.38 (t, 1 H)
MS C23H22F4N03S2 m/z 500 (M+1).
Example 2:
Figure imgf000031_0001
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)-thiazol-5- yl]ethoxy}phenoxy)propionic acid ethyl ester
To a solution of intermediate 39 ( 238 mg, 1 mmol) and intermediate 16 (287 mg, 1 mmol, 1 eq) in dry THF was added PBu3 ( 0.37mL, 1.5 mmol, 1.5 eq). The resulting mixture was stirred at 0°C, and then TMAD ( 258 mg, 1.5 mmol, 1.5 eq) was added all at once. The mixture was stirred at 0°C for 10 minutes, and at room temperature for 24 hours. The resulting mixture was filtered off and concentrated in vacuo, and the title compound was obtained as a yellow oil (270mg, 0.532 mmol) in a 53% yield after flash chromatography using cyclohexane / ethyl acetate (95:5) as eluent. MS: C26H29F3N04S m/z 507.96 (M+1)
Example 3:
Figure imgf000031_0002
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)-thiazol-5- yl]ethoxy}phenoxy)propionic acid To a solution of Example 2 (200mg, 0.394 mmol) in ethanol was added 1 N NaOH (5 mmol,
12.7 eq). The resulting mixture was stirred at 80°C for 1.5 hour, and after cooling at room temperature 1 N HCI (5 mmol, 12.7 eq) was added. The resulting mixture was concentrated in vacuo and the residue was purified by flash chromatography using CH2CI2 / MeOH (95:5) as eluent to give an oil. The product was crystallized with a mixture of water / EtOH / MeOH, filtered off and washed with water to give the title compound (50mg, 0.104 mmol) in a 26% yield.
LC/MS: C24H25F3N04S m/z 480.25 (M+1) / C24H23F3N04S: m/z 478.31 (M-1) Example 4 :
Figure imgf000032_0001
(2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}-phenoxy)-acetic acid ethyl ester A solution of ethyl (4-hydroxy-2-methylphenoxy)acetate (0.073g) and intermediate 16
(100mg) in dry tetrahydrofuran (20mL) was cooled to 0°C and tributylphosphine (0.092mg) added followed by azodicarbonyidimorpholide (0.115g) and the mixture stirred overnight. The reaction mixture was then concentrated and the residue partitioned between EtOAc (40mL) and water (40mL). The organic phase was collected, dried over magnesium sulphate and concentrated. The residue was purified by chromatography eluting with cyclohexane/ethyl acetate (10:1) to give the title compound as a colourless oil
1H NMR(CDCl3, 300 MHz) δ: 1.27 (t, 3H), 1.70 (d, 3H), ), 2.24 (s, 3H), ), 2.45 (s, 3H), 4.24 (q, 2H), 4.54 (s, 2H), 5.47 (q, 1 H), 6.60 (m, 2H), 6.75 (d, 1 H), 7.65 (d, 2H), 7.98 (d, 2H)
Example 5
Figure imgf000032_0002
(2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}-phenoxy)-acetic acid
A solution of example 4 (0.105g) in methanol (15mL) was treated with 2N NaOH (0.5mL) and the mixture heated to reflux for 20 minutes. The cooled reaction was concentrated and the residue diluted with water (20mL) 2N hydrochloric acid was added and the resulting suspension extracted with dichloromethane (20mL). The organic extract was dried over MgS0 and concentrated to give the title compound as a yellow solid.
1H NMR(CDCI3, 300Mhz) δ: 1.70 (d, 3H), 2.22 (s, 3H), 2.44 (s, 3H), 4.58 (s, 2H), 5.47 (q, 1 H), 6.60 (m, 2H), 6.75 (s, 1 H), 7.65 (d, 2H), 7.95 (d, 2H) LC/MS C22H21F3N04S m/z 452 (M+1 )
Figure imgf000032_0003
2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (3-fluoro-4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}-phenoxy)-propionic acid ethyl ester To a solution of PBu3 (460μL, 1.5 eq) and TMAD (321 mg, 1.5 eq) in dry THF(50mL) were added intermediate 39 ( 356mg, 1.4 mmol) and intermediate 20 (380mg, 1.24mmol, 1.1 eq).The resulting mixture was stirred to room temperature for 18 hrs and was concentrated to dryness to afford the title compound (270mg, 0.51mmol) as a colorless oil after flash chromatography CH2CI2/ C6H12 (80:20).
1H NMR(CDCI3) δ: 1.15 (t, 3H), 1.35(s, 6H), 1.62(d, 3H), 2.08(s, 3H), 2.34(s, 3H), 4.12(q, 2H), 5.38(q,1 H), 6.45(dd,1 H), 6.51 (d,1 H), 6.6(d,1 H), 7.52(d, 2H), 7.63(d, 2H),
Example 7:
Figure imgf000033_0001
2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (3-fluoro-4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}-phenoxy)-propionic acid
A solution of example 6 (270mg) in EtOH (15mL) was treated with 1 N sodium hydroxide
(1.54mL, 3eq.) and the mixture heated to reflux for 3 hours. The cooled reaction was concentrated and the residue diluted with water (20mL); 1 N hydrochloric acid (2mL) was added and the resulting suspension extracted with dichloromethane (20mL). The organic extract was dried over magnesium sulphate and concentrated to give the title compound in 74% yield as a yellow solid (190 mg).
1H NMR(CDCl3) δ: 1.80 (s, 6H), 1.95 (d, 3H), 2.43 (s, 3H), 2.70 (s, 3H), 5.82 (q, 1 H), 6.82
(dd, 1 H), 6.97 (m, 2H), 7.85 (m, 1 H), 7.95 (m, 2H), LC/MS: C24H22F4N04S m/z 495.7 (M-1 )
Example 8 :
Figure imgf000033_0002
(S)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}- phenoxy)-propionic acid ethyl ester The same Mitsunobu protocol as described for example 2 was applied with intermediate 18
(1.722g, 6mmol; e.e.= 95.5%) and intermediate 39 (1.43g, δmmol, 1eq.). The title compound was obtained as a pale yellow oil (1.6g, 3.15 mmol) in a 52.5% yield.
1H NMR (CDCI3, 300 MHz) δ: 1.15 (t, 3H), 1.44 (s, 6H), 1.61 (d, 3H), 2.09 (s, 3H), 2.36 (s, 3H), 4.13 (q, 2H), 5.39 (q, 1 H), 6.47 (dd, 1 H), 6.53 (d, 1 H), 6.63 (d, 1 H), 7.57 (d, 2H), 7.90 (d, 2H) Example 9 :
Figure imgf000034_0001
(S)-2-methyl-2- (2-methyl-4- {1 -[4-methyl-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}- phenoxy)-propionic acid
The same saponification protocol as for example 3 was applied usingl .6g of Example 8 (1.6g, 3.15 mmol). The white powder obtained after work up was recrystallized in Hexane / EtOH to give the title compound as a white powder (780mg, 1.628mmol) in a 52.5% yield with an e.e.= 99.9% mp 134-135°C H NMR (CDCI3) δ: 1.41 (s, 6H), 1.58 (d, 3H), 2.06 (s, 3H), 2.33 (s, 3H), 5.36 (q, 1 H), 6.46 (dd, 1 H), 6.61 (m, 2H), 7.50 (d, 2H), 7.82 (d, 2H)
[α]25 D = - 169.9 (c = 0.275, CHCI3 ) for e.e. = 99.9%
HPLC Chiralpak AD (4.6x250mm, 95% Hexane / 5% EtOH) Rt : 8.79 min
Example 10:
Figure imgf000034_0002
(R)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}- phenoxy)-propionic acid ethyl ester
The same Mitsunobu protocol as described for example 2 was applied with Intermediate 19
( 1.4g, 4.9mmol, e.e. = 98%) and intermediate 39 (1.17g, 4.9 mmol). The title compound was. obtained as a pale yellow oil (1.4g, 2.9 mmol ) in a 60% yield. 1H NMR (CDCI3) δ: 1.04 (t, 3H), 1.33 (s, 6H), 1.50 (d, 3H), 1.98 (s, 3H), 2.25 (s, 3H), 4.02
(q, 2H), 5.27 (q, 1 H), 6.35 (dd, 1 H), 6.42 (d, 1 H), 6.52 (d, 1 H), 7.46 (d, 2H), 7.80 (d, 2H)
Example 11 :
Figure imgf000034_0003
(R)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}- phenoxy)-propionic acid
The same saponification protocol as described for example 3 was applied using Example 10 ( 400mg, 0.79 mmol). The residue was purified by flash chromatography CH2CI2/ MeOH (99:1 ) to give the title compound (220mg, 0.458mmol) as a pale yellow oil in a 58% yield with an e.e.=87%
1H NMR (CDCI3, 300 MHz) δ: 1.47 (s, 6H), 1.63 (d, 3H), 2.12 (s, 3H), 2.38 (s, 3H), 5.42 (q, 1 H), 6.52 (dd, 1 H), 6.67 (m, 2H), 7.56 (d, 2H), 7.90 (d, 2H) [α]25 D= + 142 (c = 0.37, CHCI3 ) for ee = 87%
HPLC : Chiralpak-AD (4.6x250 mm, 95%Hexane / 5% EtOH ) Rt : 15 min
LC/MS: C24 H25 F3N04S m/z 480.0 (M+1)
Example 12 :
Figure imgf000035_0001
2-(4-{1-[2-(4-chloro-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl-phenoxy)-2-methyl- propionic acid ethyl ester
The same Mitsunobu conditions as described for racemic example 2 were applied to
Intermediate 23 (520mg, 2 mmol) and Phenol Intermediate 39 (530mg, 2.2mmol) and afforded in a 33% yield the title compound (320mg, 0.67 mmol) as a colorless oil. 1H NMR (CDCIg) δ: 1.15 (t, 3H), 1.44 (s, 6H), 1.60 (d, 3H), 2.09 (s, 3H), 2.33 (s, 3H), 4.13
(q, 2H), 5.36 (q, 1 H), 6.46 (dd, 1 H), 6.52 (d, 1 H), 6.62 (d, 1 H), 7.27 (d, 2H), 7.72 (d, 2H)
Example 13 :
Figure imgf000035_0002
2-(4-{1-[2-(4-chloro-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl-phenoxy)-2-methyl- propionic acid
The same saponification conditions as described for racemic example 3 were applied to example 12 (320mg, 0.67mmol) and gave in a 70% yield the title compound (210 mg, 0.47 mmol).
LC/MS :C23H23CIN04S m/z 444.1 (M-1 )
LC/MS :C23H25CIN04S m/z 446 (M+1)
Example 14 :
Figure imgf000035_0003
2-(4-{1-[2-(3,4-dichloro-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl-phenoxy)-2-methyl- propionic acid ethyl ester
The same Mitsunobu conditions as described for racemic example 2 were applied to Intermediate 22 (580mg, 2mmol) and Phenol Intermediate 39 (530mg, 2.2mmol) and afforded in a 42% yield the title compound (430mg, 0.84 mmol) as a colorless oil.
1H NMR (CDCI3) δ: 1.15 (t, 3H), 1.43 (s, 6H), 1.59 (d, 3H), 2.09 (s, 3H), 2.33 (s, 3H), 4.13 (q, 2H), 5.37 (q, 1 H), 6.46 (dd, 1 H), 6.52 (d, 1 H), 6.63 (d, 1 H), 7.36 (d, 1 H), 7.59 (dd, 1 H), 7.91 (d, 1 H)
Example 15 :
Figure imgf000036_0001
2-(4-{1-[2-(3,4-dichloro-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl-phenoxy)-2-methyl- propionic acid
The same saponification conditions as described for racemic example 3 were applied to example 14 (480mg, 0.94mmol) and gave in a 67% yield the title compound (300 mg, 0.63 mmol). LC/MS :C23H22CI2N04S m/z 478 and 480 (M-1 )
Example 16 :
Figure imgf000036_0002
2-(4-{1-[2-(4-ethyl-phenyl)-4-methyl-thiazol-5-yl1-ethoxy}-2-methyl-phenoxy)-2-methyl- propionic acid ethyl ester The same Mitsunobu conditions as described for racemic example 2 were applied to
Intermediate 21 (500mg, 2mmol) and Phenol Intermediate 39 (530mg,2.2mmol) and afforded in a 30% yield the title compound (280mg, 0.6 mmol) as a colorless oil. H NMR (CDCI3) δ: 1.25 (m, 6H), 1.53 (s, 6H), 1.70 (d, 3H), 2.19 (s, 3H), 2.42 (s, 3H), 4.23 (q, 2H), 5.46 (q,1 H), 6.57 (dd, 1 H), 6.62 (d, 1 H), 6.73 (d, 1 H), 7.23 (d, 2H), 7.81 (d, 2H)
Example 17 :
Figure imgf000036_0003
2-(4-{1-[2-(4-ethyl-phenyl)-4-methyl-thiazol-5-yl1-ethoxy}-2-methyl-phenoxy)-2-methyl- propionic acid The same saponification conditions as described for racemic example 3 were applied to example 16 (280mg, O.θmmol) and gave in a 76% yield the title compound (200 mg, 0.45 mmol). LC/MS :C25H30NO4S m/z 440.0 (M+1 )
Example 18 :
Figure imgf000037_0001
2-(4-{1-[2-(2-fluoro-4-trifluoromethyl-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl- phenoxy)-2-methyl-propionic acid ethyl ester
The same Mitsunobu conditions as described for racemic example 2 were applied to Intermediate 42 (400mg, 1.31 mmol) and Phenol Intermediate 39 (312mg, 1.31 mmol) and afforded in a 35% yield the title compound (240mg, 0.456 mmol) as a colorless oil.
1H NMR (CDCls) δ: 1.16 (t, 3H), 1.44 (s, 6H), 1.63 (d, 3H), 2.1 (s, 3H), 2.38 (s, 3H), 4.14 (q, 2H), 5.42 (q, 1 H), 6.47 (dd, 1 H), 6.52 (d, 1 H), 6.65 (d, 1 H), 7.38 (d, 2H), 8.3 (m, 1 H)
Example 19:
Figure imgf000037_0002
2-(4-{1-[2-(2-fluoro-4-trifluoromethyl-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl- phenoxy)-2-methyl-propionic acid
The same saponification conditions as described for racemic example 3 were applied to example 18 (240mg, 0.46mmol) and gave in a 97% yield the title compound as a white powder (220 mg, 0.442 mmol). mp 133°C
LC/MS :C24H22F4N04S m/z 495.9 (M-1)
Example 20 :
Figure imgf000037_0003
2-methyl-2-(2-methyl-4-{1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethoxy}-phenoxy)- propionic acid ethyl ester
The same Mitsunobu conditions as described for racemic example 2 were applied to Intermediate 17 (400mg,1.46mmol) and phenol Intermediate 39 (349mg,1.46mmol) and afforded in a 21 % yield the title compound (150mg, 0.303 mmol) as a colorless oil. 1H NMR (CDCI3) δ: 1.17 (t, 3H), 1.45 (s, 6H), 1.67 (d, 3H), 2.11 (s, 3H), 4.14 (q, 2H), 5.46 (q, 1 H), 6.55 (s, 2H), 6.69 (br s, 1 H), 7.60 (d, 2H), 7.66 (s, 1 H), 7.95 (d, 2H)
Example 21
Figure imgf000038_0001
2-methyl-2-(2-methyl-4-{1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethoxy}-phenoxy)- propionic acid
The same saponification conditions as described for racemic example 3 were applied to example 20 (150mg, 0.3mmol) and gave in a 64% yield the title compound as a yellow powder (90 mg, 0.193 mmol). LC/MS :C23H23F3N04S m/z 465.99 (M+1 )
Example 22 :
Figure imgf000038_0002
2-methyl-2-(2-methyl-4-{1-methyl-1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]- ethoxy}-phenoxy)-propionic acid ethyl ester The same Mitsunobu conditions as described for racemic example 2 were applied to
Intermediate 24 (500mg,1.66mmol) and phenol Intermediate 39 (440mg,1.85mmol) and afforded in a 70% yield the title compound (610mg, 1.17 mmol) as an oil after purification by flash chromatography CH CI2.
1H NMR (CDCI3) δ: 1.20 (t, 3H), 1.50 (s, 6H), 1.72 (s, 6H), 2.11 (s, 3H), 2.56 (s, 3H), 4.18 (q, 2H), 6.45 (dd, 1 H), 6.49 (d, 1 H), 6.64 (d, 1 H), 7.64 (d, 2H), 7.96 (d, 2H),
Example 23 :
Figure imgf000038_0003
2-methyl-2-(2-methyl-4-{1-methyl-1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]- ethoxy}-phenoxy)-propionic acid The same saponification conditions as described for racemic example 3 were applied to example 22 (300mg, 0.58mmol) and gave in a 20% yield the title compound as a yellow powder (60 mg, 0.12 mmol).
LC/MS :C25H27F3N04S m/z 494.0 (M+1)
Example 24 :
Figure imgf000039_0001
2-methyl-2-(2-methyl-4-{1-methyl-1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethoxy}- phenoxy)-propionic acid ethyl ester
The same Mitsunobu conditions as described for racemic example 2 were applied to Intermediate 25 (460mg,1.6mmol) and phenol Intermediate 39 (381 mg,1.85mmol) and afforded in a 13.5% yield the title compound (110mg, 1.17 mmol) as an oil after purification by flash chromatography C6H12 / EtOAc (80:20) 1H NMR (CDCIs) δ: 1.15 (t, 3H), 1.46 (s, 6H), 1.50 (s, 3H),1.68 (s, 6H), 2.06 (s, 3H), 4.13 (q,
2H), 5.46 (q, 1 H), 6.55 (s, 2H), 6.69 (br s, 1 H), 7.60 (d, 2H), 7.66 (s, 1 H), 7.95 (d, 2H)
Example 25 :
Figure imgf000039_0002
2-methyl-2-(2-methyl-4-{1-methyl-1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl1-ethoxy}- phenoxy)-propionic acid
The same saponification conditions as described for racemic example 3 were applied to example 24 (110mg, 0.217mmol) and gave in a 58% yield the title compound as a brown oil (60 mg, 0.125 mmol).
LC/MS :C2 H23F3N04S m/z 478.1 ( -1)
Example 26:
Figure imgf000039_0003
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)-thiazol-5- yl]propoxy}phenoxy)propionic acid ethyl ester
To a solution of intermediate 39 ( 1.03g, 4.3 mmol) and intermediate 26 (1.24g, 4.3 mmol, 1 eq) in dry toluene was added PBu3 ( 1.6mL, 6.45 mmol, 1.5 eq). The resulting mixture was allowed to stir at 0°C, and then TMAD ( 1.11g, 6.45 mmol, 1.5 eq) was added. The mixture was stirred at 0°C for 1 hour, and at room temperature for 24 hours. The resulting mixture was filtered off, concentrated in vacuo, to afford after flash chromatography ( cyclohexane/ EtOAc : 95/5) the title compound as a yellow oil (1.3g, 2.49 mmol) in a 58% yield. LC/MS: C27H31F3N04S m/z 522.19 (M+1 )
Example 27:
Figure imgf000040_0001
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)-thiazol-5- yllpropoxy}phenoxy)propionic acid
To a solution of example 26 (1.1g, 2.17 mmol) in THF / ethanol (1/1 ) was added 1 N NaOH (10 mmol, 4.6 eq). The resulting mixture was stirred at 80°C for 1.5 hour, and after cooling at room temperature 1 N HCI (10 mmol, 4.6 eq) was added. The resulting mixture was concentrated in vacuo, and the residue was sonicated in water / EtOH. The resulting solid was filtered off and washed with water , diluted HCI, and water to give the title compound (680mg, 1.38 mmol) in a
64% yield as a white powder.
LC/MS: C25H27F3N04S: m/z 493.79 (M+1 )
Example 28 :
Figure imgf000040_0002
(R)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] propoxy}- phenoxy)-propionic acid ethyl ester
The same Mitsunobu conditions as described for racemic example 2 were applied to intermediate 28 (1.4g, 4.65 mmol;e.e.= 98%) and phenol intermediate 39 (1.11g, 4.65 mmol) and gave in a 54% yield the title compound (1.3g, 2.5mmol) with an e.e. = 89%. MS : C27H31F3N04S m/z: 522.1 (M+1)
[α]25 D = + 147.5 (c = 0.268, CHCI3) for ee = 89%
HPLC : Chiralpak-AD (4.6x250 mm, 95%Hexane / 5% EtOH ) Rt : 8.98 min
Example 29 :
Figure imgf000040_0003
(R)-2-methyl-2- (2-methyl-4- {1 -f4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] propoxy}- phenoxy)-propionic acid
The same saponification conditions as described for racemic example 3 were applied to example 28 (1.245g,2.4mmol, e.e. = 89%) and gave in a 68% yield the title compound as a white powder (800 mg, 1.6mmol) with an e.e. = 89%. MS : C25H27F3N04S: m/z 494.1 (M+1 )
[α]25 D= + 145.2 (c = 0.259, CHCI3) for e.e. = 89%
HPLC : Chiralpak-AD (4.6x250 mm, 95%Hexane / 5% EtOH ) Rt : 15.88 min
Example 30 :
Figure imgf000041_0001
(S)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] propoxy}- phenoxy)-propionic acid ethyl ester
The same Mitsunobu conditions as described for racemic example 2 were applied to (R)- alcohol intermediate 30 (1.58g,5.2mmol, e.e.= 98%) and phenol intermediate 39 (1.5g, 6.24mmol) and gave in a 59% yield the title compound (1.6g, 3mmol).
1H NMR (CDCI3, 300 Mhz) δ :1.02 (t, 3H), 1.20 (t, 3H), 1.49 (s, 6H), 1.88 (m, 1 H), 2.11 (m, 1 H), 2.14 (s, 3H), 2.42 (s, 3H), 4.16 (q, 2H), 5.16 (t,1H), 6.51 (dd, 1 H), 6.56 (d, 1H), 6.67 (d, 1 H), 7.62(d, 2H), 7.96 (d, 2H),
Example 31 :
Figure imgf000041_0002
(S)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] propoxy}- phenoxy)-propionic acid
The same saponification conditions as described for racemic example 3 were applied to example 30 (1.6g, 3mmol) and gave in a 74% yield the title compound as a viscous oil (1.1g, 2.2 mmol) with an e.e. = 83%.
1H NMR(CDCI3) δ: 1.03 (t, 3H), 1.51 (s, 6H), 1.89 (m, 1 H), 2.12 (m, 1 H), 2.16 (s, 3H), 2.24 (s, 3H), 5.18 (t, 1 H), 6.55 (dd, 1 H), 6.71 (m, 2H), 7.60 (d, 2H), 7.93 (d, 2H)
[α]25 D= -129 (c = 0.322, CHCI3) for e.e. = 83%
HPLC : Chiralpak-AD (4.6x250 mm, 95%Hexane / 5% EtOH ) Rt : 7.57 min
Example 32:
Figure imgf000041_0003
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)-thiazol-5-yl]but-3- enyloxy}phenoxy)propionic acid ethyl ester
To a solution of intermediate 39 (1.5g, 6.3 mmol) and intermediate 32 (1.97g, 6.3 mmol, 1 eq) in dry toluene was added PBu, ( 2.35mL, 9.45 mmol, 1.5 eq). The resulting mixture was stirred at 0°C, and then TMAD (1.63g, 9.45 mmol, 1.5 eq) was added. The mixture was stirred at 0°C for 1 hour, and at RT for 24 hours. The resulting mixture was filtered off and concentrated in vacuo, and the title compound was obtained by flash chromatography using cyclohexane / ethyl acetate (8/2) as eluent, as a yellow oil (2.08g, 3.9 mmol) in a 62% yield.
1H NMR (CDCIs): δ 1.21 (t, 3H), 1.5 (s, 6H), 2.15 (s, 3H), 2.42 (s, 3H), 2.65 (m, 1 H), 2.85 (m, 1 H), 4.2 (q, 2H), 5.15 (m, 2H), 5.30 (t, 1 H), 5.85 (m, 1 H), 6.55 (m, 2H), 6.7 (d, 1 H), 7.65 (d, 2H), 7.97 (d, 2H),
LC/MS: C28H3ιF3N04S: m/z 534.21 (M+1 )
Example 33:
Figure imgf000042_0001
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)-thiazol-5-yl1but-3- enyloxy}phenoxy)propionic acid
To a solution of example 32 (1g, 1.87 mmol) in THF / ethanol (1/1 ) was added dropwise 0.2N NaOH (5.6 mmol, 3 eq). The resulting mixture was stirred at 50 °C for 3 hours, and after cooling at 0°C 0.2N HCI (5.6 mmol, 3eq) was added. The resulting mixture was concentrated in vacuo, then the residue was taken up in water and extracted with ethyl acetate. The organic phase was evaporated off and the residue was purified by flash chromatography using CH2CI2 / MeOH (97/3) as eluent. The residue was vigorously treated with 0.2N NaOH for 1 hour and then 0.2N HCI was added dropwise to yield to the precipitation of a white solid. After filtration, the white solid was washed with a small quantity of heptane to give the title compound (1 OOmg, 0.2 mmol) in a 11 % yield as a white powder.
LC/MS: C26H27F3N04S: m/z 505.97 (M+1) / C26H25F3N04S: m/z 504.04 (M-1)
Example 34 :
Figure imgf000042_0002
2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] butoxy}- phenoxy)-propionic acid ethyl ester
A mixture of 1 g of example 32 (1.8mmol), 100mg of 10% Pd on charcoal in EtOH (50mL) was stirred under an Hydrogen atmosphere at room temperature during 4 hours. The mixture was filtered through a celite pad and rinsed with 25 mL EtOH. The filtrate was concentrated to dryness to give the title compound (1.0g, 2mmol) as a brown powder in a quantitative crude yield.
MS : C28H33F3N04S : m/z 536.1 (M+1 ) Example 35 :
Figure imgf000043_0001
2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] butoxy}- phenoxy)-propionic acid To a solution of example 34 (800 mg, 1.5 mmol) in THF/Ethanol (5/20mL) was added 1 N
NaOH (10 mmol, 10mL). The resulting mixture was stirred at 50 °C for 2 hours, and after cooling at room temperature 1 N HCI (10 mmol, 10mL) was added. The resulting mixture was concentrated in vacuo, then the residue was flash chromatographed CH2CI2/ MeOH (98:2) to give the title compound (310 mg, 0.61 mmol) in a 40% yield as a colorless oil. MS : C26H27F3N04S : m/z 506.16 (M-1 )
MS : C26H29F3N04S : m/z 508.0 (M+1)
Example 36:
Figure imgf000043_0002
2-methyl-{2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl1- propoxy}-phenoxy)}-propionic acid ethyl ester
To a solution of phenol intermediate 39 ( 1.58 g, 6.6 mmol) and intermediate 31 (1.9g, 6.6mmol, 1.1 eq) in dry toluene was added PBu3 (1.66mL, 1.5 eq). The resulting mixture was allowed to warm to 0°C, and then TMAD (1.15g, 1.5 eq) was added. The mixture was stirred at 0°C for 1 hour, and at room temperature for 24 hours. The resulting mixture was filtered off and concentrated in vacuo, and the title compound was obtained by flash chromatography using
Cyclohexane/Ethyl acetate (95/5), as a yellow oil (450mg, 0.84 mmol) in a 14% yield. MS : C28H33F3N04S : m/z 536.1 (M+1)
Example 37 :
Figure imgf000043_0003
2-methyl-{2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl1- propoxy}-phenoxy)}-propionic acid
To a solution of example 36 (450 mg, 0.84 mmol) in THF/Ethanol (5/20mL) was added 1 N NaOH (5mmol, 5mL). The resulting mixture was stirred at 50 °C for 2 hours, and after cooling at room temperature 1 N HCI (5 mmol, 5mL) was added. The resulting mixture was concentrated in vacuo and the residue was flash chromatographed CH2CI2/ EtOAc (95:5) to give the title compound (290 mg, 0.57 mmol) in a 68 % yield as an amorphous yellow powder.
MS : C26H27F3N04S : m/z 506.24 (M-1 )
MS : C26H29F3N04S : m/z 508.1 (M+1)
Example 38:
Figure imgf000044_0001
2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl1 pentyloxy}- phenoxy)-propionic acid ethyl ester
To a solution of Intermediate 39 ( 238mg, 1 mmol) and intermediate 33 (329mg, 1 mmol, 1 eq) in dry toluene was added PBu3 (1.5 mmol, 1.5 eq). The resulting mixture was allowed to warm to 0°C, and then TMAD (1.55 mmol, 1.5 eq) was added. The mixture was stirred at 0°C for 1 hour, and at room temperature for 24 hours. The resulting mixture was filtered off and concentrated in vacuo, and the title compound was obtained by flash chromatography using Cyclohexane/EtOAc
(90:10), as a yellow oil (100mg, 0.18 mmol) in a 18 % yield. H NMR(CDCI3) δ: 0.8 (t, 3H), 1.15 (t, 3H), 1.25 (m, 2H), 1.4 (s, 6H), 1.75 (m, 1 H), 2.05 (m,
4H), 2.3 (s, 3H), 4.1 (q, 2H), 5.15 (t, 1 H), 6.45 (dd, 1 H), 6.55 (d, 1 H), 6.6 (d, 1 H), 7.55 (d, 2H), 7.9
(d, 2H)
Example 39:
Figure imgf000044_0002
2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl1 pentyloxy}- phenoxy)-propionic acid
To a solution of example 38 (90 mg, 0.164 mmol) in THF/Ethanol (1/1) was added 1 N NaOH (5 mmol, 5mL). The resulting mixture was stirred at 80 °C for 1.5 hour, and after cooling at room temperature 1 N HCI (5 mmol, 5mL) was added. The resulting mixture was concentrated in vacuo, then the residue was flash chromatographed eluting with CH2CI2/ EtOAc (90:10) to give the title compound (20 mg, 0.038 mmol) in a 23% yield as a colorless oil.
1H NMR(CDCI3) δ: 0.8 (t, 3H), 1.15-1.35 (m, 4H), 1.45 (s, 6H), 1.8 (m, 1 H), 2.05 (m, 4H), 2.35 (s, 3H); 5.15 (t, 1 H), 6.45 (m, 1 H), 6.65 (m, 2H), 7.55 (d, 2H), 7.9 (d, 2H) Example 40 :
Figure imgf000045_0001
2-(4-{cyclopentyl-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-methoxy}-2-methyl- phenoxy)-2-methyl-propionic acid ethyl ester
To a solution of Intermediate 39 ( 714mg, 3 mmol) and intermediate 34 (1.024g, 3mmol, 1.1 eq) in dry toluene was added PBu3 (1.1mL, 1.5 eq). The resulting mixture was allowed to warm to
0°C, and then TMAD (774mg, 1.5 eq) was added. The mixture was stirred at 0°C for 1 hour, and at room temperature for 24 hours. The resulting mixture was filtered off and concentrated in vacuo, and the title compound was obtained as a viscous yellow oil (950mg, 0.84 mmol) in a 56 % yield by flash chromatography using Cyclohexane/EtOAc (95:5) LC/MS : C30H35F3NO4S : m/z 562.0 (M+1 )
Example 41 :
Figure imgf000045_0002
2-(4-{cyclopentyl-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yll-methoxy}-2-methyl- phenoxy)-2-methyl-propionic acid To a solution of example 40 (930 mg, 1.66 mmol) in THF/Ethanol (10/60mL) was added dropwise 1 N NaOH (8.3mmol, 8.3mL) diluted in 15mL of water. The resulting mixture was stirred at 60 °C for 4 hours, and after cooling at room temperature 1 N HCI (9 mmol, 9mL) diluted in 20mL of water was added. The resulting mixture was concentrated in vacuo and the residue was flash chromatographed CH2CI2/ MeOH (98:2) to give the title compound (690 mg, 1.29 mmol) in a 78 % yield as a white amorphous powder.
LC/MS : C28H29F3N04S : m/z 532.2 (M-1) LC/MS : C28H31F3N04S : m/z 534.2 (M+1 )
Example 42:
Figure imgf000045_0003
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)-thiazol-5- yl]phenylmethoxy}phenoxy)propionic acid ethyl ester
To a solution of intermediate 39 (715mg, 3 mmol) and intermediate 35 (1.05g, 3 mmol, 1 eq) in dry toluene was added PBu3 (1.11 mL, 4.5 mmol, 1.5 eq). The resulting mixture was allowed to stir at 0°C, and then TMAD (775mg, 4.5 mmol, 1.5 eq) was added. The mixture was stirred at 0°C for 1 hour, and at room temperature for 24 hours. The resulting mixture was filtered off and concentrated in vacuo, and the title compound was obtained by flash chromatography using cyclohexane / ethyl acetate (85:15) as eluent, as a yellow oil (870mg, 1.53 mmol) in a 51 % yield. LC/MS: C31H31F3N04S: m/z 569.99 (M+1 )
Example 43:
Figure imgf000046_0001
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)-thiazol-5- ylphenylmethoxy}phenoxy)propionic acid
To a solution of example 42 (740mg, 1.3 mmol) in THF / ethanol (1/1) was added dropwise 0.2N NaOH (6.5 mmol, 5 eq). The resulting mixture was stirred at 50°C for 4 hours, and after cooling at 0°C 0.2N HCI (6.5 mmol, 5 eq) was added. The resulting mixture was concentrated in vacuo, then the residue was taken up in water and extracted with ethyl acetate. The organic phase was evaporated off and the residue was purified by flash chromatography using CH2CI2 / ethyl acetate (80/20) as eluent. The residue was vigorously treated with 0.2N NaOH for 1 hour and then 0.2N HCI was added dropwise to yield to the precipitation of a white solid. After filtration, the white solid was washed with a small quantity of heptane to give the title compound (300mg, 0.55 mmol) in a 43% yield.
LC/MS: C29H27F3N04S: m/z 541.94 (M+1 ) / C29H25F3N04S: m/z 540.03 (M-1 )
Example 44:
Figure imgf000046_0002
2-methyl-2-(2-methyl-4-[4-methyl-2-(4-trifluoromethylphenyl)thiazol-5-yl- methoxy]phenoxy)propionic acid ethyl ester
480mg of Intermediate 39 (2 mmol) in 50mL of acetone were stirred with 1g of Cs2C03 for 15 minutes. Intermediate 40 ( 651 mg, 2.2mmol) was added and the mixture heated to reflux for 1 hour. After filtration and concentration under vacuo the residue was taken up with 200mL of
CH2CI2 and washed with water. The organic layer was dried over Na2S04, filtered and concentrated to afford 0.9g of the expected compound as an oil.
1H NMR (CDCIs): δ 1.21 (t, 3H), 1.5 (s, 6H), 2.17 (s, 3H), 2.42 (s, 3H), 4.2 (q, 2H), 5.05 (s, 2H), 6.62 (dd, 1 H), 6.75 (m, 2H), 7.58 (d, 2H), 7.92 (d, 2H),
Figure imgf000047_0001
2-methyl-2-(2-methyl-4-[4-methyl-2-(4-trifluoromethylphenyl)thiazol-5-yl- methoxy]phenoxy)propionic acid
900mg of example 44 dissolved in 30mL of EtOH and 10mL of 1 N NaOH were heated to reflux for 1 hour. After cooling to room temperature the solution was acidified with 1 N HCI and concentrated under vacuo. Trituration of the residue with water afforded after filtration and washing with water an off white powder which was recrystallized with CH3CN to give 370mg of the title compound as slightly yellow crystals. mp: 154°C
Example 46 :
Figure imgf000047_0002
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yll-2-phenyl- ethoxy}-phenoxy)-propionic acid ethyl ester
The same Mitsunobu conditions as described for racemic example 2 were applied to Intermediate 36 (1g, 2.75mmol) and phenol Intermediate 39 (655mg, 2.75mmoI) and afforded in a
21 % yield the title compound (340mg, 0.58 mmol) as a brown residue after purification by flash chromatography C6H12/EtOAc (9:1 ).
LC/MS C32H33F3N04S m/z 584.2 (M+1 )
Example 47 :
Figure imgf000047_0003
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-2-phenyl- ethoxy}-phenoxy)-propionic acid
The same saponification conditions as described for racemic example 3 were applied to example 46 (280mg, 0.48mmol) and gave in a 79% yield the title compound (210mg, 0.37 mmol) after flash chromatography CH2CI2/MeOH (95:5) as an amorphous brown powder.
LC/MS C30H27F3NO4S m/z 554.3 (M-1 ) Example 48 :
Figure imgf000048_0001
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethylsulfanyl}- phenoxy)-propionic acid ethyl ester
To a suspension of intermediate 16 (287mg, 1 mmol) and Znl2 (160mg, O.δmmol) in 10mL CH2CI2 was added intermediate thiophenol 44 (305mg, 1.2mmol) in solution in 10 mL of CH2CI2.
After stirring 24 hours at rt the reaction was not complete and a supplementary equivalent of Znl2 (319mg, I mmol) was added. The mixture was stirred 24 hours at rt quenched with water (10 mL) and 100 mL of CH2CI2 were added and the organic phase separated dried over Na2S04 concentrated under vacuo and purified by flash chromatography C6H12/EtOAc (90:10). The title compound (320mg, 0.61 mmol) was obtained as a colorless viscous oil in a 61 % yield.
LC/MS :C26H29F3N03S2 m/z 523.98 (M+1 )
Example 49:
Figure imgf000048_0002
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl1-ethylsulfanyl}- phenoxy)-propionic acid
The same saponification conditions as described for racemic example 3 were applied to example 48 (290mg, 0.56mmol) and gave in a 47% yield the title compound as a white powder (130 mg, 0.262 mmol).
1H NMR (CDCIs) δ: 1.59 (s, 6H), 1.68 (d, 3H), 1.99 (s, 3H), 2.14 (s, 3H), 4.41 (q, 1 H), 6.61 (dd, 1 H), 6.95 (dd, 1 H), 7.04 ( br s, 1 H), 7.15 (d, 1 H), 7.66 (d, 2H), 7.94 (d, 2H)
Example 50 :
Figure imgf000048_0003
2-methyl-2-(2-methyl-4-{1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethylsulfanyl}-phenoxy)- propionic acid ethyl ester The same Mitsunobu conditions as described for racemic example 2 were applied to
Intermediate 17 (350mg,1.3mmol) and thiophenol Intermediate 44 (358mg,1.3mmol) and afforded in a 80% yield the title compound (520mg, 1.02 mmol) as an oil after purification by flash chromatography CH2CI2 1H NMR (CDCI3) δ: 1.24 (t, 3H), 1.62 (s, 6H), 1.74 (d, 3H), 2.20 (s, 3H), 4.24 (q, 2H), 4.50 (q, 1 H), 6.56 (d, 1 H), 7.07 (dd, 1 H), 7.20 (d, 1 H), 7.71 (d, 2H), 8.03 (d, 2H)
j Example 51 :
Figure imgf000049_0001
2-methyl-2-(2-methyl-4-{1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethylsulfanyl}-phenoxy)- propionic acid
The same saponification conditions as described for racemic example 3 were applied to example 50 (520mg, 1.02mmol) and gave in a quantitative yield the title compound as an off- 0 white powder.
LC/MS C23H23F3N03S2 m/z 481.93 (M+1) LC/MS C23H21F3N03S2 m/z 480.00 (M-1 )
Example 52 :
Figure imgf000049_0002
5 2-methyl-2-(2-methyl-4-{1-methyl-1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]- ethylsulfanyl}-phenoxy)-propionic acid ethyl ester
The same coupling conditions as described for example 48 were applied to Intermediate 24 (350mg,1.16mmol) and thiophenol intermediate 44 (354mg,1.3mmol) and afforded in a 22% yield the title compound (140mg, 0.26mmol) as a colorless oil after purification by flash 0 chromatography C6H12/EtOAc (80:20)
LC/MS C27H31F3N03S2 m/z 538 (M+1 )
Example 53 :
Figure imgf000049_0003
2-methyl-2-(2-methyl-4-{1-methyl-1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]- 5 ethylsulfanyl}-phenoxy)-propionic acid The same saponification conditions as described for racemic example 3 were applied to example 52 (140mg, 0.26mmol) and gave in a 52% yield the title compound (70mg,0.13mmol) as a colorless oil.
LC/MS C25H27F3N03S2 m/z 510.1 (M+1) LC/MS C25H25F3N03S2 m/z 508.2 (M-1)
Example 54:
Figure imgf000050_0001
2-methyi-2-(2-methyl-4-{1-methyl-1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethylsulfanyl}- phenoxy)-propionic acid ethyl ester
The same coupling conditions as described for example 48 were applied to Intermediate 25 (350mg,1.22mmol) and thiophenol intermediate 44 (371 mg,1.4mmol) and afforded in a 46% yield the title compound (300mg, 0.57mmol) as a colorless oil after purification by flash chromatography CH2CI2.
1H NMR (CDCI3) δ: 1.1 (t, 3H), 1.46 (s, 6H), 1.63 (s, 6H), 2.05 (s, 3H), 4.1 (q, 2H), 6.4 (d, 1 H), 6.85 (dd, 1 H), 6.98 (d, 1 H), 7.62 (d, 2H), 7.95 (d, 2H)
Example 55:
Figure imgf000050_0002
2-methyl-2-(2-methyl-4-{1-methyl-1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethylsulfanyl}- phenoxy)-propionic acid
The same saponification conditions as described for racemic example 3 were applied to example 54 (300mg, 0.54mmol) and gave in a 48% yield the title compound (130mg, 0.262mmol) as a gummy solid.
LC/MS C24H25F3N03S2 m/z 496.0 (M+1)
Example 56
Figure imgf000050_0003
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]- propylsulfanyl}-phenoxy)-propionic acid ethyl ester
The same coupling conditions as described for example 48 were applied to Intermediate 26 (800mg,2.6mmol) and thiophenol intermediate 44 (660mg,2.6mmol) and afforded in a 71 % yield the title compound (1 OOOmg, 1.86mmol) as a slightly yellow oil after purification by flash chromatography C6H 2/EtOAc (95:5).
LC/MS C27H31F3N03S2 m/z 538.01 (M+1 )
Example 57 :
Figure imgf000051_0001
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]- propylsulfanyl}-phenoxy)-propionic acid
The same saponification conditions as described for racemic example 3 were applied to example 56 (1g, 1.86mmol) and gave in a 88% yield the title compound (840mg, 1.65 mmol) as an amorphous white powder.
LC/MS C25H27F3N03S2 m/z 510.0 (M+1)
LC/MS C25H25F3N03S2 m/z 508.1 (M-1)
The following intermediates and ligands were prepared for the binding and transfection assays described below:
Figure imgf000051_0002
(i) 2-{2-methyl-4-[({4-methyl-2-r4-(trifluoromethyl)phenyll-1 ,3-thiazol-5- yl}methyl)sulfanyl]phenoxy}acetic acid.
This compound was used as a PPARdelta reference in the transfection assays described below and was prepared according to the method reported in WO200100603-A1
(ii) 2-methyl-2-[4-{[(4-methyl-2-[4-trifluoromethylphenyl]-thiazol-5-yl carbonyl)amino]methyl}-phenoxy]propionic acid.
This compound was used as a PPAR alpha reference in the transfection assay described below and was prepared according to method reported in WO200140207-A1
(iii) 5-{4-[2-(Methyl-pyridin-2-yl-amino)-ethoxy1-benzyl}-thiazolidine-2,4 -dione This compound was used as a PPAR gamma reference in the transfection assay described below and was prepared according to method reported in J.Med.Chem. 1994, 37(23), 3977
Binding Assay: Compounds were tested for their ability to bind to hPPAR gamma hPPARalpha. or
PPARdelta using a Scintillation Proximity Assay (SPA). The PPAR ligand binding domain (LBD) was expressed in E. coli as polyHis tagged fusion proteins and purified. The LBD was then labelled with biotin and immobilised on streptavidin-modified scintillation proximity beads. The beads were then incubated with a constant amount of the appropriate radioligand (3H-BRL 49653 for PPARgamma, radiolabelled 2-(4-(2-(2,3-Ditritio-1-heptyl-3-(2,4- difluorophenyI)ureido)ethyI)phenoxy)-2-methylbutanoic acid for hPPAR alpha (see WO 00/08002.) and labelled GW 2433 (see Brown, P. J et al . Chem. Biol., 4, 909-918 (1997). For the structure and synthesis of this ligand) for PPAR delta) and variable concentrations of test compound, and after equilibration the radioactivity bound to the beads was measured by a scintillation counter. The amount of nonspecific binding, as assessed by control wells containing 50 μM of the corresponding unlabeled ligand, was subtracted from each data point. For each compound tested, plots of ligand concentration vs. CPM of radioligand bound were constructed and apparent Kl values were estimated from nonlinear least squares fit of the data assuming simple competitive binding. The details of this assay have been reported elsewhere (see, Blanchard, S. G. et. al. Development of a Scintillation Proximity Assay for Peroxisome Proliferator-Activated Receptor gamma Ligand Binding Domain. Anal. Biochem., 257, 112-119 (1998)).
Transfection assay:
Compounds were screened for functional potency in transient transfection assays in CV-1 cells for their ability to activate the PPAR subtypes (transactivation assay). A previously established chimeric receptor system was utilized to allow comparison of the relative transcriptional activity of the receptor subtypes on the same target gene and to prevent endogenous receptor activation from complicating the interpretation of results. See, for example, Lehmann, J. M.; Moore, L. B.; Smith-Oliver, T. A.; Wilkison, W. O.; Willson, T. M.; Kliewer, S. A., An antidiabetic thiazolidinedione is a high affinity ligand for peroxisome proliferator-activated receptor gamma (PPARgammaJ, J. Biol. Chem., 270, 12953-6 (1995). The ligand binding domains for murine and human PPAR alpha, PPAR gamma, and PPAR delta were each fused to the yeast transcription factor GAL4 DNA binding domain. CV-1 cells were transiently transfected with expression vectors for the respective PPAR chimera along with a reporter construct containing five copies of the GAL4 DNA binding site driving expression of secreted placental alkaline phosphatase (SPAP) and beta-galactosidase. After 16 h, the medium was exchanged to DME medium supplemented with 10% delipidated fetal calf serum and the test compound at the appropriate concentration. After an additional 24h, cell extracts were prepared and assayed for alkaline phosphatase and β-galactosidase activity. Alkaline phosphatase activity was corrected for transfection efficiency using the beta-galactosidase activity as an internal standard (see, for example, Kliewer, S. A., et. al. Cell 83, 813-819 (1995)). Rosiglitazone (BRL 49653) was used as a positive control in the hPPAR gamma assay. The positive control in the hPPAR alpha assays was 2-methyl-2-[4-{[(4-methyl-2-[4-trifluoromethylphenyl]-thiazol-5-yl-carbonyl)amino]methyl}- phenoxy]propionic acid. The positive control for PPAR delta assays was 2-{2-methyl-4-[({4- methyl-2-{trifluoromethyl)phenyl]-1 ,3-thiazol-5-yl}methyl)sulfanyl]phenoxy}acetic acid.
All of the above acid Examples showed at least 50% activation of hPPARδ relative to the positive control at concentrations of 10"7 M or less.
Activities in three hPPAR subtypes are reported in the table below for the most preferred compounds and are expressed in nanomolar.
Example EC50 hPPAR α EC50 hPPAR δ EC50 hPPAR γ
1 43 2.5 57
9 16 3 7000
11 10 0.7 300
31 5 1.7 660
29 9 1.5 240

Claims

What is claimed is:
1. A compound of formula (I) or a pharmaceutically acceptable salt, solvate, or hydrolysable ester thereof, wherein:
Figure imgf000054_0001
(1) R1 and R2 are independently H or C-ι.3 alkyl, or R1 and R2 which are bonded to the same carbon atom may together with the carbon atom to which they are bonded form a 3-6 membered cycloalkyl ring, and at least one of R1 and R2 must be other than H; X2 is O, S, or (CR10R11)n where n is 1 or 2;
R3, R4, and R5 are independently H, C^alkyl, OCH3, CF3, allyl, or halogen; R10 and R11 are independently H, fluorine, or Chalky!; one of Y and Z is N, the other is S or O;
R6 and R7 are independently H, phenyl, benzyl, fluorine OH, Cι-6 alkyl, allyl, R6 and R7 may, together with the carbon atom to which they are bonded, represent a carbonyl group;
R9 is H, CF3 or CH3;
Each R8 is independently CF3, Chalky], OCH3 or halogen; y is O, 1 , 2, 3, 4, or 5.
2. A compound according to claim 1 wherein at least one of R1 and R2 is CH3.
3. A compound according to claim 2 wherein R1 and R2 are both CH3.
4. A compound according to any of claims 1-3 wherein X2 is O, S, or C(R10R11).
5. A compound according to claim 4 wherein X2 is O or S.
6. A compound according to any of claims 1-5 wherein Z is N.
7. A compound according to any of claims 1-6 wherein Y is S.
8. A compound according to any of claims 1-8 wherein R3 is CH3 and R4 and R5 are independently H or CH3.
9. A compound according to any preceding claim wherein R6 is H, CH2CH3, or CH3.
10. A compound according to any preceding claim wherein R7 is H.
11. A compound according to any of claims 1 -10 wherein y is 1 or 2.
12. A compound according to claim 11 wherein y is 2.
13. A compound according to claim 12 wherein the one of R8 substituents is halogen.
14. A compound according to claim 13 wherein one of the R8 substituents is halogen and the other is CF3.
15. A compound according to claim 10 wherein y is 1.
16. A compound according to claim 15 wherein the R8 substituent is in the para position.
17. A compound according to claim 18 wherein R8 is CF3.
18. A compound according to claim 1 selected from the group consisting of: 2-{4-[({2-[2-fluoro-4-(trifluoromethyl)phenyl]-4-methyl-1 ,3-thiazol-5-yl}methyl)suIfanyl]-2- methylphenoxy}-2-methylpropanoic acid
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)thiazol-5- yl]ethoxy}phenoxy)propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)thiazol-5- yl]propoxy}phenoxy)propionic acid ethyl ester 2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)thiazol-5-yl]but-3- enyloxy}phenoxy)propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)-thiazol-5- yl]phenylmethoxy}phenoxy)propionic acid ethyl ester
(2-methyI-4- {1 -[4-methyl-2- (4-trifluoromethylphenyl)-thiazoI-5-yl] ethoxy}-phenoxy)-acetic acid ethyl ester
2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (3-Fluoro-4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}-phenoxy)-propionic acid ethyl ester (S)-2-methyl-2- (2-methyl-4- {1 -[4-methyl-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}- phenoxy)-propionic acid ethyl ester
(R)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}- phenoxy)-propionic acid ethyl ester 2-(4-{1-[2-(4-chloro-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl-phenoxy)-2-methyl- propionic acid ethyl ester
2-(4-{1-t2-(3,4-dichloro-phenyl)-4-methyl-thiazol-5-yI]-ethoxy}-2-methyl-phenoxy)-2-methyl- propionic acid ethyl ester
2-(4-{1-[2-(4-ethyl-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl-phenoxy)-2-methyl- propionic acid ethyl ester
2-(4-{1-[2-(2-fluoro-4-trifluoromethyl-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl- phenoxy)-2-methyl-propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethoxy}-phenoxy)- propionic acid ethyl ester 2-methyl-2-(2-methyl-4-{1-methyl-1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yI]- ethoxy}-phenoxy)-propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-methyI-1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethoxy}- phenoxy)-propionic acid ethyl ester
(R)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] propoxy}- phenoxy)-propionic acid ethyl ester
(S)-2-methyl-2- (2-methyI-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] propoxy}- phenoxy)-propionic acid ethyl ester
2-methyl-2- (2-methyl-4- {1-[4-methyI-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] butoxy}- phenoxy)-propionic acid ethyl ester 2-methyl-{2-methyl-2- (2-methyI-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyI)-thiazol-5-yl]- propoxy}-phenoxy)}-propionic acid ethyl ester
2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] pentyloxy}- phenoxy)-propionic acid ethyl ester
2-(4-{cyclopentyl-[4-methyl-2-(4-trifluoromethyI-phenyl)-thiazol-5-yl]-methoxy}-2-methyl- phenoxy)-2-methyl-propionic acid ethyl ester
2-methyl-2-(2-methyl-4-[4-methyl-2-(4-trifluoromethylphenyl)thiazol-5-yl- methoxy]phenoxy)propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-2-phenyl- ethoxy}-phenoxy)-propionic acid ethyl ester 2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethylsulfanyl}- phenoxy)-propionic acid ethyl ester 2-methyl-2-(2-methyl-4-{1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethyIsulfanyl}-phenoxy)- propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-methyl-1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yI]- ethylsulfanyI}-phenoxy)-propionic acid ethyl ester 2-methyl-2-(2-methyl-4-{1-methyI-1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethyisulfanyl}- phenoxy)-propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]- propylsulfanyl}-phenoxy)-propionic acid ethyl ester
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)-thiazol-5- yl]phenylmethoxy}phenoxy)propionic acid
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyIphenyl)thiazol-5- yl]ethoxy}phenoxy)propionic acid
2-methyI-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)thiazoI-5- yl]propoxy}phenoxy)propionic acid 2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethylphenyl)-thiazol-5-yl]but-3- enyloxy}phenoxy)propionic acid
(2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}-phenoxy)-acetic acid
2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (3-Fluoro-4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}-phenoxy)-propionic acid
(S)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyl)- hiazol-5-yl] ethoxy}- phenoxy)-propionic acid
(R)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}- phenoxy)-propionic acid 2-(4-{1-[2-(4-chIoro-phenyl)-4-methyl-thiazoi-5-yl]-ethoxy}-2-methyl-phenoxy)-2-methyI- propionic acid
2-(4-{1-[2-(3,4-dichIoro-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl-phenoxy)-2-methyl- propionic acid
2-(4-{1-[2-(4-ethyl-phenyl)-4-methyI-thiazol-5-yl]-ethoxy}-2-methyl-phenoxy)-2-methyl- propionic acid
2-(4-{1-[2-(2-fluoro-4-trifluoromethyl-phenyl)-4-methyl-thiazol-5-yl]-ethoxy}-2-methyl- phenoxy)-2-methyl-propionic acid
2-methyl-2-(2-methyl-4-{1-[2-(4-trifluoromethyl-phenyl)-thiazoi-5-yl]-ethoxy}-phenoxy)- propionic acid 2-methyl-2-(2-methyl-4-{1-methyl-1-[4-methyi-2-(4-trifluoromethyi-phenyl)-thiazol-5-yl]- ethoxy}-phenoxy)-propionic acid 2-methyl-2-(2-methyl-4-{1-methyl-1-[2-(4-trifluoromethyI-phenyl)-thiazol-5-yl]-ethoxy}- phenoxy)-propionic acid
(R)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] propoxy}- phenoxy)-propionic acid (S)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yI] propoxy}- phenoxy)-propionic acid
2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyI)-thiazol-5-yl] butoxy}- phenoxy)-propionic acid
2-methyl-{2-methyl-2- (2-methyl-4- {1 -[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl]- propoxy}-phenoxy)}-propionic acid
2-methyI-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] pentyloxy}- phenoxy)-propionic acid
2-(4-{cyclopentyl-[4-methyI-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-methoxy}-2-methyl- phenoxy)-2-methyl-propionic acid 2-methyl-2-(2-methyI-4-[4-methyl-2-(4-trifluoromethylphenyl)thiazoi-5-yl- methoxy]phenoxy)propionic acid
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyI-phenyl)-thiazol-5-yl]-2-phenyl- ethoxy}-phenoxy)-propionic acid
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethylsulfanyl}- phenoxy)-propionic acid
2-methyl-2-(2-methyl-4-{1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethyIsulfanyl}-phenoxy)- propionic acid
2-methyl-2-(2-methyl-4-{1-methyl-1-[4-methyl-2-(4-trifluoromethyI-phenyl)-thiazol-5-yl]- ethylsulfanyl}-phenoxy)-propionic acid 2-methyl-2-(2-methyl-4-{1-methyl-1-[2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]-ethylsulfanyl}- phenoxy)-propionic acid
2-methyl-2-(2-methyl-4-{1-[4-methyl-2-(4-trifluoromethyl-phenyl)-thiazol-5-yl]- propylsulfanyl}-phenoxy)-propionic acid and pharmaceutically acceptable salts and solvates thereof.
19. A compound according to claim 1 selected from the group consisting of:
2-{4-[({2-t2-fluoro-4-(trifluoromethyl)pheny)]-4-methyl-1 ,3-thiazol-5-yl}methyl)sulfanyl]-2- methylphenoxy}-2-methylpropanoic acid
(S)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}- phenoxy)-propionic acid
(R)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethylphenyl)-thiazol-5-yl] ethoxy}- phenoxy)-propionic acid (R)-2-methyl-2- (2-methyl-4- {1-[4-methyi-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] propoxy}- phenoxy)-propionic acid
(S)-2-methyl-2- (2-methyl-4- {1-[4-methyl-2- (4-trifluoromethyl-phenyl)-thiazol-5-yl] propoxy}- phenoxy)-propionic acid and pharmaceutically acceptable salts and solvates thereof.
20. A compound according to any preceding claim which is a hPPAR agonist.
21. A compound according to any of claims 1 -20 for use in therapy.
22. A pharmaceutical composition comprising a compound according to any of claims 1 - 20.
23. Use of a compound according to any of claims 1 -20 for the manufacture of a medicament for the treatment of a hPPAR disease or condition.
24. Use according to claim 23 wherein the hPPAR mediated disease or condition is dyslipidemia, syndrome X, heart failure, hypercholesteremia, cardiovascular disease, type II diabetes mellitus, type I diabetes, insulin resistance, hyperiipidemia, obesity, anorexia bulimia and anorexia nervosa
25. A method of treating a hPPAR mediated disease or condition in a patient comprising the administration of a therapeutically effective amount of a compound according to any of claims 1-20.
26. A method according to claim 25 wherein the hPPAR mediated disease or condition is dyslipidemia, syndrome X, heart failure, hypercholesteremia, cardiovascular disease, type II diabetes mellitus, type I diabetes, insulin resistance, hyperiipidemia, obesity, anorexia bulimia and anorexia nervosa.
PCT/US2001/049230 2000-12-20 2001-12-19 Thiazole derivatives for treating ppar related disorders WO2002062774A1 (en)

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