WO2007136876A3 - Delta-5 desaturase from peridinium sp. and its use in making polyunsaturated fatty acids - Google Patents

Delta-5 desaturase from peridinium sp. and its use in making polyunsaturated fatty acids Download PDF

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Publication number
WO2007136876A3
WO2007136876A3 PCT/US2007/012232 US2007012232W WO2007136876A3 WO 2007136876 A3 WO2007136876 A3 WO 2007136876A3 US 2007012232 W US2007012232 W US 2007012232W WO 2007136876 A3 WO2007136876 A3 WO 2007136876A3
Authority
WO
WIPO (PCT)
Prior art keywords
desaturase
delta
fatty acids
polyunsaturated fatty
peridinium
Prior art date
Application number
PCT/US2007/012232
Other languages
French (fr)
Other versions
WO2007136876A2 (en
Inventor
Howard G Damude
Quinn Qun Zhu
Original Assignee
Du Pont
Howard G Damude
Quinn Qun Zhu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Howard G Damude, Quinn Qun Zhu filed Critical Du Pont
Priority to MX2008014471A priority Critical patent/MX2008014471A/en
Priority to CA2647198A priority patent/CA2647198C/en
Priority to EP07809151A priority patent/EP2019866A2/en
Priority to BRPI0711028-6A priority patent/BRPI0711028A2/en
Publication of WO2007136876A2 publication Critical patent/WO2007136876A2/en
Publication of WO2007136876A3 publication Critical patent/WO2007136876A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)

Abstract

Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-5 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this delta-5 desaturase in plants and oleaginous yeast are disclosed.
PCT/US2007/012232 2006-05-17 2007-05-17 Delta-5 desaturase from peridinium sp. and its use in making polyunsaturated fatty acids WO2007136876A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
MX2008014471A MX2008014471A (en) 2006-05-17 2007-05-17 Delta-5 desaturase from peridinium sp. and its use in making polyunsaturated fatty acids.
CA2647198A CA2647198C (en) 2006-05-17 2007-05-17 Delta-5 desaturase and its use in making polyunsaturated fatty acids
EP07809151A EP2019866A2 (en) 2006-05-17 2007-05-17 Delta-5 desaturase from peridinium sp. and its use in making polyunsaturated fatty acids
BRPI0711028-6A BRPI0711028A2 (en) 2006-05-17 2007-05-17 "isolated polynucleotide, recombinant DNA construct, cell, method for transforming a cell, method for producing a transgenic plant, transgenic seeds, long chain polyunsaturated fatty acid production method, oils or subporudots to produce, at least one Polyunsaturated Fatty Acid in a Vegetable Cell of an Oilseed, Plants and Isolated Nucleic Acid Molecule "

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US80111906P 2006-05-17 2006-05-17
US60/801,119 2006-05-17

Publications (2)

Publication Number Publication Date
WO2007136876A2 WO2007136876A2 (en) 2007-11-29
WO2007136876A3 true WO2007136876A3 (en) 2008-07-10

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PCT/US2007/011717 WO2007136646A2 (en) 2006-05-17 2007-05-16 Delta5 desaturase and its use in making polyunsaturated fatty acids
PCT/US2007/012232 WO2007136876A2 (en) 2006-05-17 2007-05-17 Delta-5 desaturase from peridinium sp. and its use in making polyunsaturated fatty acids

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PCT/US2007/011717 WO2007136646A2 (en) 2006-05-17 2007-05-16 Delta5 desaturase and its use in making polyunsaturated fatty acids

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US (3) US7695950B2 (en)
EP (2) EP2018427B1 (en)
CN (1) CN101448946A (en)
AT (1) ATE544858T1 (en)
BR (1) BRPI0711028A2 (en)
CA (1) CA2647198C (en)
DK (1) DK2018427T3 (en)
MX (1) MX2008014471A (en)
RU (1) RU2008149700A (en)
WO (2) WO2007136646A2 (en)
ZA (1) ZA200807830B (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7709239B2 (en) * 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
US8957280B2 (en) * 2007-05-03 2015-02-17 E. I. Du Pont De Nemours And Company Delta-5 desaturases and their use in making polyunsaturated fatty acids
US8168858B2 (en) 2008-06-20 2012-05-01 E. I. Du Pont De Nemours And Company Delta-9 fatty acid elongase genes and their use in making polyunsaturated fatty acids
DK2324119T3 (en) 2008-09-19 2016-07-18 Du Pont Mutant DELTA5 Desaturases AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids
ES2466365T3 (en) * 2008-10-06 2014-06-10 Abbott Laboratories Delta-8 desaturase genes, enzymes encoded by them and uses thereof
CN102186876B (en) 2008-10-14 2014-06-11 孟山都技术公司 Utilization of fatty acid desaturases from hemiselmis spp.
JP5620402B2 (en) 2008-12-18 2014-11-05 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ Reduction of malonic acid by-product formation in the fermentation process
US20110178105A1 (en) 2010-01-15 2011-07-21 E.I. Du Pont De Nemours And Company Clinical benefits of eicosapentaenoic acid in humans
CN103282474A (en) 2010-04-22 2013-09-04 纳幕尔杜邦公司 Method for obtaining polyunsaturated fatty acid-ontaining compositions from microbial biomass
US20120204802A1 (en) 2010-08-11 2012-08-16 E. I. Du Pont De Nemours And Company Sustainable aquaculture feeding strategy
US20120040076A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
CA2805882A1 (en) 2010-08-11 2012-02-16 E. I. Dupont De Nemours And Company Improved aquaculture meat products
EP2609202A1 (en) 2010-08-26 2013-07-03 E.I. Du Pont De Nemours And Company Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids
CN103443266A (en) 2010-12-30 2013-12-11 纳幕尔杜邦公司 Use of saccharomyces cerevisiae SUC2 gene in yarrowia lipolytica for sucrose utilization
CA3026436A1 (en) 2011-02-11 2012-08-16 E. I. Du Pont De Nemours And Company Method for obtaining a lipid-containing composition from microbial biomass
US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
US8980591B2 (en) * 2011-08-04 2015-03-17 Suntory Holdings Limited Protein having activity to promote fatty acid chain elongation, gene encoding same and use thereof
CN104195148B (en) * 2014-09-16 2016-08-10 合肥工业大学 A kind of pavlova viridis delta-5 delta 8 desaturase genes and preparation method for EPA synthesis
US10530712B2 (en) 2016-12-09 2020-01-07 Cisco Technology, Inc. Handling reflexive ACLs with virtual port-channel
CN112410355B (en) * 2020-11-23 2022-03-25 昆明理工大学 Acyl-coenzyme A oxidase 2 gene RKACOX2 and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002057464A2 (en) * 2001-01-19 2002-07-25 Basf Plant Science Gmbh Method for the expression of biosynthetic genes in plant seeds using multiple expression constructs
WO2004087902A2 (en) * 2003-03-31 2004-10-14 University Of Bristol Novel plant acyltransferases specific for long-chained, multiply unsaturated fatty acids

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4948811A (en) * 1988-01-26 1990-08-14 The Procter & Gamble Company Salad/cooking oil balanced for health benefits
PL336077A1 (en) 1997-04-11 2000-06-05 Calgene Llc Methods of and compositions for synthesising long-chain polyunsaturated fatty acids in plants
US6432684B1 (en) 1997-04-11 2002-08-13 Abbott Laboratories Human desaturase gene and uses thereof
US5968809A (en) * 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US6051754A (en) * 1997-04-11 2000-04-18 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
US5972664A (en) 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US6075183A (en) 1997-04-11 2000-06-13 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
US6403349B1 (en) 1998-09-02 2002-06-11 Abbott Laboratories Elongase gene and uses thereof
US6677145B2 (en) 1998-09-02 2004-01-13 Abbott Laboratories Elongase genes and uses thereof
US6825017B1 (en) * 1998-12-07 2004-11-30 Washington State University Research Foundation Desaturases and methods of using them for synthesis of polyunsaturated fatty acids
NZ512656A (en) 1998-12-07 2003-09-26 Univ Washington Desaturases and methods of using them for synthesis of polyunsaturated fatty acids
WO2002000905A2 (en) 2000-06-28 2002-01-03 Monsanto Technology Llc Plant regulatory sequences for selective control of gene expression
SI1309698T1 (en) 2000-07-21 2007-08-31 Du Pont A cytochrome p450 enzyme associated with the synthesis of delta-12 -epoxy groups in fatty acids of plants
MXPA03006663A (en) 2001-01-25 2004-05-31 Abbott Lab Desaturase genes and uses thereof.
GB0107510D0 (en) 2001-03-26 2001-05-16 Univ Bristol New elongase gene and a process for the production of -9-polyunsaturated fatty acids
US7045683B2 (en) 2001-05-04 2006-05-16 Abbott Laboratories Δ4-desaturase genes and uses thereof
US7211656B2 (en) * 2002-01-30 2007-05-01 Abbott Laboratories Desaturase genes, enzymes encoded thereby, and uses thereof
US7714185B2 (en) 2002-12-19 2010-05-11 University Of Bristol Method for the production of polyunsaturated fatty acids
US20040172682A1 (en) 2003-02-12 2004-09-02 Kinney Anthony J. Production of very long chain polyunsaturated fatty acids in oilseed plants
EP1599582B1 (en) * 2003-02-27 2011-07-27 BASF Plant Science GmbH Method for the production of polyunsaturated fatty acids
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7259255B2 (en) 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
BRPI0413073A (en) * 2003-08-01 2006-10-03 Basf Plant Science Gmbh process for the production of compounds, oil, lipid or fatty acid, or a fraction thereof, oil, lipid or fatty acid composition, process for the production of oils, lipids or fatty acid compositions, use of oil, lipid or acids fatty acids or compositions of oil, lipid or fatty acid or oils, lipids or fatty acid compositions, isolated nucleic acid sequence, amino acid sequence, gene construct, vector, and transgenic non-human organism
JP4916886B2 (en) 2003-11-12 2012-04-18 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー Δ15 desaturase suitable for altering polyunsaturated fatty acid levels in oily plants and yeast
US7504259B2 (en) 2003-11-12 2009-03-17 E. I. Du Pont De Nemours And Company Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
US7202356B2 (en) 2003-11-14 2007-04-10 E. I. Du Pont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast
CN103451246B (en) * 2004-04-22 2018-02-16 联邦科学技术研究组织 With recombinant cell synthesis of long-chain polyunsaturated fatty acids
US7834250B2 (en) 2004-04-22 2010-11-16 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
PT1766023E (en) * 2004-06-04 2010-12-09 Fluxome Sciences As Metabolically engineered cells for the production of polyunsaturated fatty acids
US7550651B2 (en) 2004-06-25 2009-06-23 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7189559B2 (en) 2004-11-04 2007-03-13 E. I. Du Pont De Nemours And Company Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7198937B2 (en) 2004-11-04 2007-04-03 E. I. Du Pont De Nemours And Company Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7192762B2 (en) 2004-11-04 2007-03-20 E. I. Du Pont De Nemours And Company Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7550286B2 (en) 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
US7126672B2 (en) * 2004-12-27 2006-10-24 Asml Netherlands B.V. Lithographic apparatus and device manufacturing method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002057464A2 (en) * 2001-01-19 2002-07-25 Basf Plant Science Gmbh Method for the expression of biosynthetic genes in plant seeds using multiple expression constructs
WO2004087902A2 (en) * 2003-03-31 2004-10-14 University Of Bristol Novel plant acyltransferases specific for long-chained, multiply unsaturated fatty acids

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHEN CHIH-YU ET AL: "Screening of red algae filaments as a potential alternative source of eicosapentaenoic acid", MARINE BIOTECHNOLOGY (NEW YORK), vol. 4, no. 2, March 2002 (2002-03-01), pages 189 - 192, XP002478174, ISSN: 1436-2228 *
DATABASE EMBL [online] 1 November 2004 (2004-11-01), "Sequence 56 from Patent WO2004087902.", retrieved from EBI accession no. EMBL:CQ891290 Database accession no. CQ891290 *
DATABASE EPO Proteins [online] 16 August 2002 (2002-08-16), "Sequence 31 from Patent WO02057464.", retrieved from EBI accession no. EPOP:AX481970 Database accession no. AX481970 *
SHAMSUDIN L: "Lipid and fatty acid contents in red tides from tropical fish ponds of the coastal water of South China Sea", ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY, vol. 104, no. 1, 1996, pages 36 - 42, XP009099380, ISSN: 1381-3455 *

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Publication number Publication date
EP2018427B1 (en) 2012-02-08
US8049070B2 (en) 2011-11-01
US20080286436A1 (en) 2008-11-20
CA2647198C (en) 2016-01-12
MX2008014471A (en) 2008-11-26
EP2019866A2 (en) 2009-02-04
ATE544858T1 (en) 2012-02-15
US20120076894A1 (en) 2012-03-29
RU2008149700A (en) 2010-06-27
DK2018427T3 (en) 2012-05-29
CA2647198A1 (en) 2007-11-29
WO2007136876A2 (en) 2007-11-29
CN101448946A (en) 2009-06-03
ZA200807830B (en) 2009-12-30
US7695950B2 (en) 2010-04-13
BRPI0711028A2 (en) 2012-08-14
EP2018427A2 (en) 2009-01-28
WO2007136646A3 (en) 2008-03-20
WO2007136646A2 (en) 2007-11-29
US20070271632A1 (en) 2007-11-22

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