WO2011084627A3 - Modified cry1ca insecticidal cry proteins - Google Patents

Modified cry1ca insecticidal cry proteins Download PDF

Info

Publication number
WO2011084627A3
WO2011084627A3 PCT/US2010/060826 US2010060826W WO2011084627A3 WO 2011084627 A3 WO2011084627 A3 WO 2011084627A3 US 2010060826 W US2010060826 W US 2010060826W WO 2011084627 A3 WO2011084627 A3 WO 2011084627A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
dig
cry proteins
cry1ca
methods
Prior art date
Application number
PCT/US2010/060826
Other languages
French (fr)
Other versions
WO2011084627A2 (en
Inventor
Thomas Meade
Kenneth Narva
Timothy Hey
Ignacio Larrinua
Aaron T. Woosley
Stephanie L. Burton
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to JP2012544843A priority Critical patent/JP2013514772A/en
Priority to US13/516,622 priority patent/US9284573B2/en
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Priority to CN2010800640116A priority patent/CN102762096A/en
Priority to AU2010339916A priority patent/AU2010339916B2/en
Priority to RU2012130019/10A priority patent/RU2012130019A/en
Priority to KR1020127018297A priority patent/KR101854478B1/en
Priority to NZ601094A priority patent/NZ601094A/en
Priority to UAA201208626A priority patent/UA112286C2/en
Priority to CA2782554A priority patent/CA2782554A1/en
Priority to EP10842617.2A priority patent/EP2512224A4/en
Priority to BRBR112012015005-7A priority patent/BR112012015005A2/en
Priority to MX2012007124A priority patent/MX345224B/en
Publication of WO2011084627A2 publication Critical patent/WO2011084627A2/en
Publication of WO2011084627A3 publication Critical patent/WO2011084627A3/en
Priority to IL220335A priority patent/IL220335A/en
Priority to ZA2012/04918A priority patent/ZA201204918B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal protein (delta-endotoxin)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

The present invention includes modified, insecticidal B.t. Cry1Ca proteins, including the proteins designated herein as DIG-109 and DIG-152, as well as variants of DIG-109 and DIG-152, nucleic acids encoding these proteins, methods of controlling pests using the proteins, methods of producing the proteins in transgenic host cells, and transgenic plants that produce the proteins. The DIG-109 and DIG-152 proteins comprise chimeric peptides composed of a core toxin segment of B.t. Cry1Ca and a Cry1Ab protoxin segment. Insecticidally active variants of the DIG-109 and DIG-152 proteins are also described.
PCT/US2010/060826 2009-12-16 2010-12-16 Modified cry1ca insecticidal cry proteins WO2011084627A2 (en)

Priority Applications (14)

Application Number Priority Date Filing Date Title
CA2782554A CA2782554A1 (en) 2009-12-16 2010-12-16 Modified cry1ca insecticidal cry proteins
UAA201208626A UA112286C2 (en) 2009-12-16 2010-12-16 MODIFIED INSECTICIDIC CRY1C PROTEIN AND METHOD FOR FIGHTING INSULATED INSECTS WITH ITS USE
CN2010800640116A CN102762096A (en) 2009-12-16 2010-12-16 Modified cry1ca insecticidal cry proteins
US13/516,622 US9284573B2 (en) 2009-12-16 2010-12-16 Modified Cry1Ca insecticial Cry proteins
RU2012130019/10A RU2012130019A (en) 2009-12-16 2010-12-16 MODIFIED INSECTICIDAL Cry-PROTEINS Cry1Ca
KR1020127018297A KR101854478B1 (en) 2009-12-16 2010-12-16 MODIFIED CRY1Ca INSECTICIDAL CRY PROTEINS
EP10842617.2A EP2512224A4 (en) 2009-12-16 2010-12-16 Modified cry1ca insecticidal cry proteins
JP2012544843A JP2013514772A (en) 2009-12-16 2010-12-16 Modified Cry1Ca insecticidal Cry protein
AU2010339916A AU2010339916B2 (en) 2009-12-16 2010-12-16 Modified Cry1CA insecticidal Cry proteins
NZ601094A NZ601094A (en) 2009-12-16 2010-12-16 Modified cry1ca insecticidal cry proteins
BRBR112012015005-7A BR112012015005A2 (en) 2009-12-16 2010-12-16 Modified cry1ca insecticidal cry proteins
MX2012007124A MX345224B (en) 2009-12-16 2010-12-16 Modified cry1ca insecticidal cry proteins.
IL220335A IL220335A (en) 2009-12-16 2012-06-12 Modified cry1ca insecticidal cry proteins
ZA2012/04918A ZA201204918B (en) 2009-12-16 2012-07-02 Modified cry1ca insecticidal cry proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28427509P 2009-12-16 2009-12-16
US61/284,275 2009-12-16

Publications (2)

Publication Number Publication Date
WO2011084627A2 WO2011084627A2 (en) 2011-07-14
WO2011084627A3 true WO2011084627A3 (en) 2011-12-22

Family

ID=44306062

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/060826 WO2011084627A2 (en) 2009-12-16 2010-12-16 Modified cry1ca insecticidal cry proteins

Country Status (18)

Country Link
US (1) US9284573B2 (en)
EP (1) EP2512224A4 (en)
JP (1) JP2013514772A (en)
KR (1) KR101854478B1 (en)
CN (1) CN102762096A (en)
AR (1) AR079502A1 (en)
AU (1) AU2010339916B2 (en)
BR (1) BR112012015005A2 (en)
CA (1) CA2782554A1 (en)
CL (1) CL2012001631A1 (en)
CO (1) CO6592053A2 (en)
IL (1) IL220335A (en)
MX (1) MX345224B (en)
NZ (1) NZ601094A (en)
RU (1) RU2012130019A (en)
UA (1) UA112286C2 (en)
WO (1) WO2011084627A2 (en)
ZA (1) ZA201204918B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106459158A (en) * 2014-04-29 2017-02-22 美国陶氏益农公司 Monoclonal antibodies specific for Cry1Ca and related detection methods

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2015004179A (en) * 2012-10-05 2015-06-10 Dow Agrosciences Llc Use of cry1ea in combinations for management of resistant fall armyworm insects.
CN103145814B (en) * 2013-02-25 2014-07-09 北京大北农科技集团股份有限公司 Insecticidal protein, and coding gene and use thereof
BR112016018287A2 (en) * 2014-02-07 2017-10-10 Du Pont insecticide proteins and methods for their use
TW201542093A (en) 2014-03-21 2015-11-16 艾格里遺傳學股份有限公司 Cry1D for controlling corn earworm
EA034764B9 (en) * 2014-10-16 2020-05-21 Монсанто Текнолоджи Ллс LEPIDOPTERAN-ACTIVE Cry1Da1 AMINO ACID SEQUENCE VARIANT PROTEINS
US10570182B2 (en) * 2014-12-30 2020-02-25 Dow Agrosciences Llc Modified Cry1Ca toxins useful for control of insect pests
WO2016186986A1 (en) * 2015-05-19 2016-11-24 Pioneer Hi Bred International Inc Insecticidal proteins and methods for their use
US10412369B2 (en) 2015-07-31 2019-09-10 Dell Products, Lp Method and apparatus for compensating for camera error in a multi-camera stereo camera system
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CN109781993B (en) * 2018-12-27 2021-04-06 中国农业科学院生物技术研究所 Enzyme linked immunosorbent assay kit for quantitatively detecting insect-resistant protein Cry1C
AU2020226376A1 (en) * 2019-02-20 2021-08-05 Syngenta Crop Protection Ag Engineered pesticidal proteins and methods of controlling plant pests
CN111100208A (en) * 2020-01-16 2020-05-05 黑龙江大鹏农业有限公司 Artificially synthesized insect-resistant protein mCry1Ia2, and preparation method and application thereof
CN111574599B (en) * 2020-05-18 2022-10-28 福建农林大学 Toxin modification method for solving excessive enzymolysis of insecticidal toxin by insect intestinal digestive enzyme

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US5380831A (en) * 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US20030101482A1 (en) * 1996-11-27 2003-05-29 Monsanto Technology Llc Compositions encoding lepidopteran-toxic polypeptides and methods of use
WO2003082910A1 (en) * 2002-03-27 2003-10-09 Council Of Scientific And Industrial Research Chimeric delta endotoxin protein of cry1ea and cry1ca
US20060288448A1 (en) * 2005-06-08 2006-12-21 Pioneer Hi-Bred International, Inc. Insect-specific protease recognition sequences

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US5380831A (en) * 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US20030101482A1 (en) * 1996-11-27 2003-05-29 Monsanto Technology Llc Compositions encoding lepidopteran-toxic polypeptides and methods of use
WO2003082910A1 (en) * 2002-03-27 2003-10-09 Council Of Scientific And Industrial Research Chimeric delta endotoxin protein of cry1ea and cry1ca
US20060288448A1 (en) * 2005-06-08 2006-12-21 Pioneer Hi-Bred International, Inc. Insect-specific protease recognition sequences

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Publication number Priority date Publication date Assignee Title
CN106459158A (en) * 2014-04-29 2017-02-22 美国陶氏益农公司 Monoclonal antibodies specific for Cry1Ca and related detection methods

Also Published As

Publication number Publication date
ZA201204918B (en) 2013-02-27
JP2013514772A (en) 2013-05-02
RU2012130019A (en) 2014-01-27
AU2010339916B2 (en) 2016-04-21
IL220335A (en) 2017-01-31
CL2012001631A1 (en) 2013-01-25
AU2010339916A1 (en) 2012-07-12
NZ601094A (en) 2014-10-31
MX2012007124A (en) 2012-11-12
US20130025006A1 (en) 2013-01-24
US9284573B2 (en) 2016-03-15
CN102762096A (en) 2012-10-31
KR101854478B1 (en) 2018-05-03
EP2512224A2 (en) 2012-10-24
MX345224B (en) 2017-01-20
EP2512224A4 (en) 2013-04-10
BR112012015005A2 (en) 2015-09-01
KR20120101546A (en) 2012-09-13
UA112286C2 (en) 2016-08-25
WO2011084627A2 (en) 2011-07-14
CA2782554A1 (en) 2011-07-14
CO6592053A2 (en) 2013-01-02
AR079502A1 (en) 2012-02-01

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